CREB Regulated Transcription Coactivator 1 Recombinant Protein | CRTC1 recombinant protein

Recombinant CREB Regulated Transcription Coactivator 1 (CRTC1)

Cat. Num. AAA2034595

• Gene Names: CRTC1; MECT1; TORC1; TORC-1; WAMTP1
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: CREB Regulated Transcription Coactivator 1; Recombinant CREB Regulated Transcription Coactivator 1 (CRTC1); Target Of Rapamycin Complex; Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1; CRTC1 recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Human
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 634

• Applicable Applications for CRTC1 recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Met1~Ser172
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Met1~Ser172 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20380067
• NCBI GeneID: 23373
• Molecular Weight: Predicted: 22.9kDa
• NCBI Official Full Name: CREB regulated transcription coactivator 1
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 1
• NCBI Official Symbol: CRTC1
• NCBI Official Synonym Symbols: MECT1; TORC1; TORC-1; WAMTP1
• NCBI Protein Information: CREB-regulated transcription coactivator 1
• UniProt Protein Name: CREB-regulated transcription coactivator 1
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC1
• UniProt Synonym Gene Names: TORC-1; Transducer of CREB protein 1
• UniProt Entry Name: CRTC1_HUMAN

Uniprot Description

TORC1: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long- term potentiation (L-LTP) maintenance at the Schaffer collateral- CA1 synapses. May be required for dendritic growth of developing cortical neurons. Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Binds HTLV1 Tax. Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. Belongs to the TORC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane

Molecular Function: cAMP response element binding protein binding; protein binding

Biological Process: activation of CREB transcription factor; entrainment of circadian clock by photoperiod; positive regulation of transcription from RNA polymerase II promoter