CRNN recombinant protein

Recombinant Human CRNN Protein

Cat. Num. AAA8248838

• Gene Names: CRNN; DRC1; PDRC1; SEP53; C1orf10
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: CRNN; Recombinant Human CRNN Protein; C1orf10; DRC1; PDRC1; SEP53; Cornulin; 53 kDa putative calcium-binding protein; 53 kDa squamous epithelial-induced stress protein; 58 kDa heat shock protein; Squamous epithelial heat shock protein 53; Tumor-related protein; CRNN recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: MGSSHHHHHH SSGLVPRGSH MPQLLQNING IIEAFRRYAR TEGNCTALTR GELKRLLEQE FADVIVKPHD PATVDEVLRL LDEDHTGTVE FKEFLVLVFK VAQACFKTLS ESAEGACGSQ ESGSLHSGAS QELGEGQRSG TEVGRAGKGQ HYEGSSHRQS
• Sequence Length: 495

• Applicable Applications for CRNN recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CRNN recombinant protein

Recombinant Human CRNN Protein is produced by our E.coli expression system and the target gene encoding Met1-Ser140 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7706635
• NCBI GeneID: 49860
• NCBI Accession #: NP_057274.1
• NCBI GenBank Nucleotide #: NM_016190.2
• UniProt Accession #: Q9UBG3; Q8N613; B2RE60
• Molecular Weight: 53,533 Da
• NCBI Official Full Name: cornulin
• NCBI Official Synonym Full Names: cornulin
• NCBI Official Symbol: CRNN
• NCBI Official Synonym Symbols: DRC1; PDRC1; SEP53; C1orf10
• NCBI Protein Information: cornulin
• UniProt Protein Name: Cornulin
• Protein Family: Cornulin
• UniProt Gene Name: CRNN
• UniProt Synonym Gene Names: C1orf10; DRC1; PDRC1; SEP53
• UniProt Entry Name: CRNN_HUMAN

NCBI Description

This gene encodes a member of the "fused gene" family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. The encoded protein contains two EF-hand Ca2+ binding domains in its N-terminus and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. This gene, also known as squamous epithelial heat shock protein 53, may play a role in the mucosal/epithelial immune response and epidermal differentiation. [provided by RefSeq, Jan 2009]

Uniprot Description

CRNN: Survival factor that participates in the clonogenicity of squamous esophageal epithelium cell lines, attenuates deoxycholic acid (DCA)-induced apoptotic cell death and release of calcium. When overexpressed in oral squamous carcinom cell lines, regulates negatively cell proliferation by the induction of G1 arrest. Belongs to the S100-fused protein family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; membrane

Biological Process: cell-cell adhesion; response to heat