PDGFRA recombinant protein

PDGFRA Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123473

• Gene Names: Pdgfra; APDGFR; PDGFACE
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PDGFRA; PDGFRA Protein; Rat; Recombinant (His Tag); Rat PDGFRa/CD140a Protein (His Tag); platelet-derived growth factor receptor; alpha polypeptide; PDGFRA recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu523

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat PDGFRA (NP_036934.1)(Met1-Glu523) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDGFRA recombinant protein

Background: PDGFRA, also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.

Product Categories/Family for PDGFRA recombinant protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Oseini AM, et al. (2009) PDGFRalpha: a new therapeutic target in the treatment of hepatocellular carcinoma Expert Opin Ther Targets. 13(4): 443-54.
Meister M, et al. (2009) Expression and mutational status of PDGFR in thymic tumours. Anticancer Res. 29(10): 4057-61.
Martinho O, et al. (2009) Expression, mutation and copy number analysis of platelet-derived growth factor receptor A (PDGFRA) and its ligand PDGFA in gliomas. Br J Cancer. 101(6): 973-82.

NCBI and Uniprot Product Information

• NCBI GI #: 158186636
• NCBI GeneID: 25267
• NCBI Accession #: NP_036934.1
• NCBI GenBank Nucleotide #: NM_012802.1
• UniProt Accession #: P20786
• Molecular Weight: 122,642 Da
• NCBI Official Full Name: platelet-derived growth factor receptor alpha
• NCBI Official Synonym Full Names: platelet derived growth factor receptor alpha
• NCBI Official Symbol: Pdgfra
• NCBI Official Synonym Symbols: APDGFR; PDGFACE
• NCBI Protein Information: platelet-derived growth factor receptor alpha
• UniProt Protein Name: Platelet-derived growth factor receptor alpha
• Protein Family: Platelet-derived growth factor receptor
• UniProt Gene Name: Pdgfra
• UniProt Synonym Gene Names: PDGF-R-alpha; PDGFR-alpha
• UniProt Entry Name: PGFRA_RAT

NCBI Description

acts as a receptor tyrosine kinase for PDGF; may play a role in glial cell generation [RGD, Feb 2006]

Uniprot Description

PDGFRA: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation. Particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. Chromosomal rearrangments activate PDGFRalpha by fusion to BCR, causing atypical chronic myelogenous leukemia (CML), and to FIP1L1, causing idiopathic hypereosinophilic syndrome. Activating point mutations cause a minority of gastrointestinal stromal tumors (GIST). Promoter polymorphisms linked to neural tube defects including spina bifida, verified by mouse mutant model. Inhibitors: Gleevec, Sutent. OMIM: Two alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Oncoprotein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; PDGFR family

Cellular Component: cell surface; cytoplasm; cytosol; external side of plasma membrane; integral to membrane; integral to plasma membrane; intrinsic to plasma membrane; membrane; microvillus; nucleus; plasma membrane; protein complex

Molecular Function: ATP binding; phosphoinositide 3-kinase binding; platelet-derived growth factor alpha-receptor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein complex binding; protein dimerization activity; protein homodimerization activity; protein kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: adrenal gland development; anatomical structure morphogenesis; cardiac myofibril assembly; cell migration; chemotaxis; elevation of cytosolic calcium ion concentration; embryonic cranial skeleton morphogenesis; embryonic digestive tract morphogenesis; embryonic skeletal morphogenesis; estrogen metabolic process; extracellular matrix organization and biogenesis; female gonad development; gliogenesis; hemopoietic progenitor cell differentiation; in utero embryonic development; inner ear development; Leydig cell differentiation; lung development; luteinization; male genitalia development; odontogenesis of dentine-containing teeth; organ morphogenesis; palate development; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of phosphoinositide 3-kinase activity; protein amino acid autophosphorylation; regulation of chemotaxis; response to cytokine stimulus; response to estradiol stimulus; response to estrogen stimulus; response to hormone stimulus; response to hyperoxia; response to inorganic substance; response to organic substance; skeletal morphogenesis; wound healing