CRIP2 recombinant protein

CRIP2 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121712

• Gene Names: CRIP2; CRIP; CRP2; ESP1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CRIP2; CRIP2 Protein; Human; Recombinant (His & GST Tag); Human CRIP2 Protein (His & GST Tag); CRIP Protein; CRP2 Protein; ESP1 Protein; cysteine-rich protein 2; CRIP2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro208

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human CRIP2 (P52943)(Met1-Pro208) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CRIP2 recombinant protein

Background: CRIP2 is a putative transcription factor. It has a widespread tissue expression and is highly expressed in heart. CRIP2 contains two LIM zinc-binding domains. CRIP2 may participate in the differentiation of smooth muscle tissue. It also plays an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. CRIP2 acts as a transcription repressor of the NF-kappaB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis. It interacts with the NF-kappaB/p65 to inhibit its DNA-binding ability to the promoter regions of the major proangiogenesis cytokines critical for tumor progression, including IL6, IL8, and VEGF. In conclusion, we provide compelling evidence that CRIP2 acts as a transcription repressor of the NF-kappaB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis.

References

Chang DF. et al., 2003, Dev Cell. 4 (1):107-18. Huber A. et al., 2000, J Biol Chem. 275 (8): 5504-11. Karim MA. et al., 1996, Genomics. 31 (2): 167-76.

NCBI and Uniprot Product Information

• NCBI GI #: 399498490
• NCBI GeneID: 1397
• NCBI Accession #: NP_001257766
• NCBI GenBank Nucleotide #: NM_001312
• UniProt Accession #: P52943; A1A4U1; B7Z6C0; E9PD13
• Molecular Weight: 30,221 Da
• NCBI Official Full Name: cysteine-rich protein 2 isoform 2
• NCBI Official Synonym Full Names: cysteine-rich protein 2
• NCBI Official Symbol: CRIP2
• NCBI Official Synonym Symbols: CRIP; CRP2; ESP1
• NCBI Protein Information: cysteine-rich protein 2; Cysteine-rich intestinal protein; LIM domain protein ESP1/CRP2
• UniProt Protein Name: Cysteine-rich protein 2
• Protein Family: Cysteine-rich protein
• UniProt Gene Name: CRIP2
• UniProt Synonym Gene Names: CRP2; CRP-2
• UniProt Entry Name: CRIP2_HUMAN