CREB Binding Protein (CREBBP) Recombinant Protein | CREBBP recombinant protein

Recombinant CREB Binding Protein (CREBBP)

Cat. Num. AAA2009142

• Gene Names: Crebbp; CBP; KAT3A; AW558298; CBP/p300; p300/CBP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: CREB Binding Protein (CREBBP); Recombinant CREB Binding Protein (CREBBP); CREBBP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- QQNSAS LAGGMAGHSQ FQQPQGPGGY APAMQQQRMQ QHLPIQGSSM GQMAAPMGQL GQMGQPGLGA DSTPNIQQAL QQRILQQQQM KQQIGSPGQP NPMSPQQHML SGQPQASHLP GQQIATSLSN QVRSPAPVQS PRPQSQPPHS SPSPRIQPQP SPHHVSPQTG SPHPGLAVTM ASSMDQGHLG NPEQSAMLPQ LNTPNRSALS SELSLVGDTT GDTLEKFVEG L
• Sequence Length: 2441

• Applicable Applications for CREBBP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln2215~Leu2441 (Accession # P45481) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 341940549
• NCBI GeneID: 12914
• UniProt Accession #: P45481; E9QPH4
• Molecular Weight: 25.4kDa
• NCBI Official Full Name: CREB-binding protein
• NCBI Official Synonym Full Names: CREB binding protein
• NCBI Official Symbol: Crebbp
• NCBI Official Synonym Symbols: CBP; KAT3A; AW558298; CBP/p300; p300/CBP
• NCBI Protein Information: CREB-binding protein
• UniProt Protein Name: CREB-binding protein
• Protein Family: CREB-binding protein
• UniProt Gene Name: Crebbp
• UniProt Synonym Gene Names: Cbp
• UniProt Entry Name: CBP_MOUSE

Uniprot Description

CBP: a protein acetyltransferase that can transcriptionally activate histones. Acetylates the NCOA3 coactivator. Binds specifically to phosphorylated CREB1 and enhances its transcriptional activity toward cAMP-responsive genes. Methylation of the KIX domain by CARM1 blocks association with CREB, blocking CREB signaling, and activating the apoptotic response. Found in a complex containing NCOA2, NCOA3, IKKA, IKKB, and IKBKG. Probably part of a complex with HIF1A and EP300. Interacts with the C-terminal region of CITED4. The TAZ-type 1 domain interacts with HIF1A. Interacts with MAF, SRCAP, CARM1, ELF3, MLLT7/FOXO4, N4BP2, NCOA1, NCOA3, NCOA6, PCAF, PELP1, PML, SMAD1, SMAD2, SMAD3, SPIB and TRERF1. Interacts with HTLV-1 Tax, p30II, and HIV-1 Tat. Interacts with KLF1; the interaction results in acetylation of KLF1 and enhancement of its transcriptional activity. Interacts with ZCCHC12. Interacts with DAXX; the interaction is dependent on CBP sumoylation and results in suppression of the transcriptional activiy via recruitment of HDAC2 to DAAX. Interacts with MTDH. Interacts with NFATC4. Interacts with MAFG; the interaction acetylates MAFG in the basic region and stimulates NFE2 transcriptional activity through increasing its DNA-binding activity. Interacts with IRF2; the interaction acetylates IRF2 and regulates its activity on the H4 promoter. Interacts via its N-terminus with the C-terminus of SS18L1. Interacts with MECOM. Chromosomal aberrations involving CBP may be a cause of acute myeloid leukemias. Known translocation partners include MYST3, MLL, and MYST4. MYST3-CBP fusion proteins may induce leukemia by inhibiting RUNX1-mediated transcription. Defects in CBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1), an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.

Protein type: Motility/polarity/chemotaxis; EC 2.3.1.48; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Acetyltransferase; DNA-binding

Cellular Component: nucleoplasm; nuclear body; PML body; transcription factor complex; protein complex; cytoplasm; nuclear chromatin; outer kinetochore of condensed chromosome; nucleus; histone acetyltransferase complex

Molecular Function: peroxisome proliferator activated receptor binding; transcription activator binding; zinc ion binding; p53 binding; metal ion binding; transcription coactivator activity; transcription factor binding; transferase activity; protein binding; MRF binding; histone acetyltransferase activity; DNA binding; acetyltransferase activity; transcription cofactor activity; transferase activity, transferring acyl groups; chromatin binding; SMAD binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; rhythmic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; germ-line stem cell maintenance; histone acetylation; negative regulation of transcription from RNA polymerase II promoter; N-terminal peptidyl-lysine acetylation