CREB Binding Protein (CREBBP) Recombinant Protein | CREBBP recombinant protein

Recombinant CREB Binding Protein (CREBBP)

Cat. Num. AAA2009703

• Gene Names: CREBBP; CBP; RSTS; KAT3A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: CREB Binding Protein (CREBBP); Recombinant CREB Binding Protein (CREBBP); CREBBP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- NLNAMQAGVP RPGVPPQQQA MGGLNPQGQA LNIMNPGHNP NMASMNPQYR EMLRRQLLQQ QQQQQQQQQQ QQQQQQGSAG MAGGMAGHGQ FQQPQGPGGY PPAMQQQQRM QQHLPLQGSS MGQMAAQMGQ LGQMGQPGLG ADSTPNIQQA LQQRILQQQQ MKQQIGSPGQ PNPMSPQQHM LSGQPQASHL PGQQIATSLS NQVRSPAPVQ SPRPQSQPPH SSPSPRIQPQ PSPHHVSPQT GSPHPGLAVT MASSIDQGHL GNPEQSAMLP QLNTPSRSAL SSELSLVGDT TGDTLEKFVE GL
• Sequence Length: 2404

• Applicable Applications for CREBBP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn2141~Leu2442 (Accession # Q92793) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119943102
• NCBI GeneID: 1387
• NCBI Accession #: NP_001073315.1
• NCBI GenBank Nucleotide #: NM_001079846.1
• UniProt Accession #: Q92793; O00147; Q16376; Q4LE28; D3DUC9
• Molecular Weight: 33.8kDa
• NCBI Official Full Name: CREB-binding protein isoform b
• NCBI Official Synonym Full Names: CREB binding protein
• NCBI Official Symbol: CREBBP
• NCBI Official Synonym Symbols: CBP; RSTS; KAT3A
• NCBI Protein Information: CREB-binding protein
• UniProt Protein Name: CREB-binding protein
• Protein Family: CREB-binding protein
• UniProt Gene Name: CREBBP
• UniProt Synonym Gene Names: CBP
• UniProt Entry Name: CBP_HUMAN

NCBI Description

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Uniprot Description

CBP: a protein acetyltransferase that can transcriptionally activate histones. Acetylates the NCOA3 coactivator. Binds specifically to phosphorylated CREB1 and enhances its transcriptional activity toward cAMP-responsive genes. Methylation of the KIX domain by CARM1 blocks association with CREB, blocking CREB signaling, and activating the apoptotic response. Found in a complex containing NCOA2, NCOA3, IKKA, IKKB, and IKBKG. Probably part of a complex with HIF1A and EP300. Interacts with the C-terminal region of CITED4. The TAZ-type 1 domain interacts with HIF1A. Interacts with MAF, SRCAP, CARM1, ELF3, MLLT7/FOXO4, N4BP2, NCOA1, NCOA3, NCOA6, PCAF, PELP1, PML, SMAD1, SMAD2, SMAD3, SPIB and TRERF1. Interacts with HTLV-1 Tax, p30II, and HIV-1 Tat. Interacts with KLF1; the interaction results in acetylation of KLF1 and enhancement of its transcriptional activity. Interacts with ZCCHC12. Interacts with DAXX; the interaction is dependent on CBP sumoylation and results in suppression of the transcriptional activiy via recruitment of HDAC2 to DAAX. Interacts with MTDH. Interacts with NFATC4. Interacts with MAFG; the interaction acetylates MAFG in the basic region and stimulates NFE2 transcriptional activity through increasing its DNA-binding activity. Interacts with IRF2; the interaction acetylates IRF2 and regulates its activity on the H4 promoter. Interacts via its N-terminus with the C-terminus of SS18L1. Interacts with MECOM. Chromosomal aberrations involving CBP may be a cause of acute myeloid leukemias. Known translocation partners include MYST3, MLL, and MYST4. MYST3-CBP fusion proteins may induce leukemia by inhibiting RUNX1-mediated transcription. Defects in CBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1), an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; EC 2.3.1.48; DNA-binding; Transcription, coactivator/corepressor; Acetyltransferase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; nuclear body; transcription factor complex; cytoplasm; nuclear chromatin; outer kinetochore of condensed chromosome; nucleus; histone acetyltransferase complex

Molecular Function: protein binding; MRF binding; signal transducer activity; histone acetyltransferase activity; zinc ion binding; p53 binding; acetyltransferase activity; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; establishment and/or maintenance of chromatin architecture; viral reproduction; positive regulation of transcription, DNA-dependent; rhythmic process; germ-line stem cell maintenance; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of transcription, DNA-dependent; homeostatic process; response to hypoxia; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; gene expression; protein complex assembly; embryonic digit morphogenesis; histone acetylation; N-terminal peptidyl-lysine acetylation; regulation of smoothened signaling pathway

Disease: Rubinstein-taybi Syndrome 1