Tar DNA Binding Protein 43kDa (TDP43) Recombinant Protein | TDP43 recombinant protein

Recombinant Tar DNA Binding Protein 43kDa (TDP43)

Cat. Num. AAA2010406

• Gene Names: Tardbp; Tdp43; C85084; TDP-43; 1190002A23Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tar DNA Binding Protein 43kDa (TDP43); Recombinant Tar DNA Binding Protein 43kDa (TDP43); TDP43 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- SDLIVLG LPWKTTEQDL KDYFSTFGEV LMVQVKKDLK TGHSKGFGFV RFTEYETQVK VMSQRHMIDG RWCDCKLPNS KQSPDEPLRS RKVFVGRCTE DMTAEELQQF FCQYGEVVDV FIPKPFRAFA FVTFADDKVA QSLCGEDLII KGISVHISNA EP
• Sequence Length: 414

• Applicable Applications for TDP43 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser104~Pro262 (Accession # Q921F2) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21704096
• NCBI GeneID: 230908
• NCBI Accession #: NP_663531.1
• NCBI GenBank Nucleotide #: NM_145556.4
• UniProt Accession #: Q921F2; Q3U591; Q3V0E7
• Molecular Weight: 19.7kDa
• NCBI Official Full Name: TAR DNA-binding protein 43 isoform 1
• NCBI Official Synonym Full Names: TAR DNA binding protein
• NCBI Official Symbol: Tardbp
• NCBI Official Synonym Symbols: Tdp43; C85084; TDP-43; 1190002A23Rik
• NCBI Protein Information: TAR DNA-binding protein 43
• UniProt Protein Name: TAR DNA-binding protein 43
• UniProt Gene Name: Tardbp
• UniProt Synonym Gene Names: Tdp43; TDP-43
• UniProt Entry Name: TADBP_MOUSE

Uniprot Description

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; nucleic acid binding; mRNA 3'-UTR binding; RNA binding; double-stranded DNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; positive regulation of transcription from RNA polymerase II promoter; mRNA processing