Rabbit SAFB Polyclonal Antibody | anti-SAFB antibody

SAFB Polyclonal Antibody

Cat. Num. AAA3009833

• Gene Names: SAFB; HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SAFB; Polyclonal Antibody; SAFB Polyclonal Antibody; HSP27 estrogen response element-TATA box-binding protein; HAP; HET; SAFB1; SAF-B1; anti-SAFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SAFB polyclonal antibody detects endogenous levels of SAFB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 914

• Applicable Applications for anti-SAFB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: A synthetic peptide of human SAFB
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of U-2 OS cells using SAFB antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using SAFB antibody.)

Related Product Information for anti-SAFB antibody

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 1002095852
• NCBI GeneID: 6294
• NCBI Accession #: NP_001307501.1
• NCBI GenBank Nucleotide #: NP_001307501.1
• UniProt Accession #: Q15424
• Molecular Weight: 130kDa
• NCBI Official Full Name: scaffold attachment factor B1 isoform 6
• NCBI Official Synonym Full Names: scaffold attachment factor B
• NCBI Official Symbol: SAFB
• NCBI Official Synonym Symbols: HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
• NCBI Protein Information: scaffold attachment factor B1
• UniProt Protein Name: Scaffold attachment factor B1
• Protein Family: Scaffold attachment factor
• UniProt Gene Name: SAFB
• UniProt Synonym Gene Names: HAP; HET; SAFB1; SAF-B; SAF-B1; HSP27 ERE-TATA-binding protein

NCBI Description

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (). Can inhibit cell proliferation.