Typical Testing Data/Standard Curve (for reference only)
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Mouse Cyclooxygenase 2 ELISA Kit | COX2 elisa kit
Mouse Cyclooxygenase 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: COX-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for COX-2. Standards or samples are then added to the microtiter plate wells and COX-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of COX-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for COX-2 are added to each well to "sandwich" the COX-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain COX-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX-2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Uniprot Description
COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
Protein type: EC 1.14.99.1; Oxidoreductase; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 1q25.2-q25.3
Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus
Molecular Function: prostaglandin-endoperoxide synthase activity; protein homodimerization activity; enzyme binding; peroxidase activity; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding
Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; decidualization; sensory perception of pain; prostaglandin biosynthetic process; negative regulation of synaptic transmission, dopaminergic; positive regulation of synaptic transmission, glutamatergic; response to estradiol stimulus; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; negative regulation of smooth muscle contraction; regulation of blood pressure; arachidonic acid metabolic process; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; response to fructose stimulus; water-soluble vitamin metabolic process; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; prostaglandin metabolic process; hair cycle; ovulation; response to manganese ion; NAD metabolic process; regulation of inflammatory response; positive regulation of vasoconstriction; brown fat cell differentiation; positive regulation of fever; response to oxidative stress; cell motility; embryo implantation
Research Articles on COX2
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Product Notes
The Mouse COX2 ptgs2 (Catalog #AAA720812) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720812 ELISA Kit recognizes Mouse COX2. It is sometimes possible for the material contained within the vial of "Cyclooxygenase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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