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Immunohistochemistry (IHC) (Biotinylated (0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

CTDSP1 Antibody | anti-CTDSP1 antibody

Goat anti-CTDSP1, Biotinylated Antibody

Gene Names
CTDSP1; NIF3; SCP1; NLIIF; NLI-IF
Reactivity
Human, Mouse
Expected from sequence similarity: Rat, Dog, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CTDSP1; Goat anti-CTDSP1; Biotinylated Antibody; Goat Anti-CTDSP1 Antibody; CTD small phosphatase 1; NIF3; NLI-IF; NLIIF; SCP1; CTD (carboxy-terminal domain; RNA polymerase II; polypeptide A) small phosphatase 1; NLI-interacting factor 3; nuclear LIM interactor-interacting factor 3; small C-terminal domain phosphatase 1; anti-CTDSP1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Expected from sequence similarity: Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
261
Applicable Applications for anti-CTDSP1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:16000.
WB: Approx 30kDa band observed in Mouse Testis lysates. Recommended concentration: 0.1-0.3ug/ml.
Immunogen Sequence
Peptide with sequence EAKAQDSDKIC., from the internal region of the protein sequence according to NP_067021.1; NP_872580.1; NP_001193807.1.
Peptide Sequence + C
EAKAQDSDKIC.
Epitope
Internal Region
Note
This antibody is expected to recognize all three reported isoforms (NP_067021.1; NP_872580.1; NP_001193807.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Biotinylated (0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

Immunohistochemistry (IHC) (Biotinylated (0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29.3kDa
NCBI Official Full Name
carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 isoform 1
NCBI Official Synonym Full Names
CTD small phosphatase 1
NCBI Official Symbol
CTDSP1
NCBI Official Synonym Symbols
NIF3; SCP1; NLIIF; NLI-IF
NCBI Protein Information
carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
UniProt Protein Name
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
UniProt Gene Name
CTDSP1
UniProt Synonym Gene Names
NIF3; NLIIF; SCP1; NLI-IF; NLI-interacting factor 3; SCP1; Small CTD phosphatase 1

NCBI Description

This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.

Research Articles on CTDSP1

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Product Notes

The CTDSP1 ctdsp1 (Catalog #AAA423708) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CTDSP1, Biotinylated Antibody reacts with Human, Mouse Expected from sequence similarity: Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CTDSP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Pep-ELISA: antibody detection limit dilution 1:16000. WB: Approx 30kDa band observed in Mouse Testis lysates. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CTDSP1 ctdsp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTDSP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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