ELISA (EIA)
(ELISA plate was coated by N protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
COVID 19 Nucleocapsid (NP) N3 fragment (C-ter domain) Coronavirus Recombinant Protein | COVID-19 recombinant protein
2019-nCov Nucleocapsid Recombinant Protein N3 fragment (C-ter domain)
Applications
ELISA
Purity
Purity: >95% by SDS-pagePurification: Ni column
Synonyms
COVID 19 Nucleocapsid (NP) N3 fragment (C-ter domain) Coronavirus; 2019-nCov Nucleocapsid Recombinant Protein N3 fragment (C-ter domain); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); COVID-19 recombinant protein
Host
E Coli
Specificity
No non-specific binding to normal human serum was identified so far.
Purity/Purification
Purity: >95% by SDS-page
Purification: Ni column
Purification: Ni column
Form/Format
Liquid; 20mM Tris, 300mM NaCl, PH8.5
Concentration
1.0 mg/mL (varies by lot)
Applicable Applications for COVID-19 recombinant protein
ELISA (EIA)
Product Note
According to previous study on SARS CoV, SARS NP C-ter domain can react with all SARS positive serum. We therefore designed 2019-nCoV NP C-ter domain for the good of a better IVD product. We believe the 2019-nCoV NP C-ter domain is more stable than the full-length NP since it lacks the autolysis sites. This property may make the 2019-nCoV NP C-ter domain more suitable to the IVD applications.
Preparation and Storage
Store at 2-8°C. Avoiding freeze/thaw cycles.
ELISA (EIA)
(ELISA plate was coated by N protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
ELISA (EIA)
(ELISA plate was coated by N protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
Product Categories/Family for COVID-19 recombinant protein
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Product Notes
The COVID-19 (Catalog #AAA8574728) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Nucleocapsid (NP) N3 fragment (C-ter domain) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) N3 fragment (C-ter domain) Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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