COVID 19 Neutralizing Coronavirus ELISA Kit | COVID-19 elisa kit
Anti-SARS-CoV-2 Neutralizing Antibody (NtAb) ELISA Kit
Synonyms
COVID 19 Neutralizing Coronavirus; Anti-SARS-CoV-2 Neutralizing Antibody (NtAb) ELISA Kit; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Neutralizing Antibody; Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody (NtAb) ELISA Kit; Anti-SARS-CoV-2 Spike RBD-ACE2 Binding Blocking Antibody ELISA Kit; Antibody-mediated Blockage of Spike RBD-ACE2 Protein-Protein Interaction ELISA Kit; Receptor Binding Domain; COVID-19 elisa kit
Samples
Serum, plasma
Assay Type
Qualitative Competitive
Preparation and Storage
Store at 2-8 degree C for 6 months. 1. Store Positive Control, Negative Control and HRP conjugated S-RBD antibody at -20 degree C. 2. It is recommended to use the kit within 1 month once open.
Related Product Information for COVID-19 elisa kit
Principle of the Assay: The kit is based on the competitive ELISA, it is a blocking ELISA detection tool, which mimics the virus neutralization process. The kit contains two key components: HRP conjugated recombinant SARS-CoV-2 Spike RBD fragment protein (HRP-SRBD) and the human ACE2 receptor protein (hACE2). The protein-protein interactions between HRP-SRBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2 Spike RBD. Incubate the samples and controls with HRP-SRBD to make the combination of the neutralization antibodies with HRP-SRBD. Then, add the mixture to the hACE2 pre-coated plate. The unbound HRP-SRBD as well as any HRP-SRBD bound to non-neutralizing antibody will be captured on the plate, while the neutralizing antibodies-HRP-SRBD complexes remain in the supernatant and were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is inverse to the Anti-SARS-CoV-2 Neutralizing Antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Anti-SARS-CoV-2 Neutralizing Antibody can be calculated.
Background/Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, or 2019-nCoV) is an enveloped non-segmented positive-sense RNA virus. It is the cause of coronavirus disease 2019 (COVID-19), which is contagious in humans. SARS-CoV-2 has several structural proteins including spike (S), envelope (E),membrane (M) and nucleocapsid (N). The spike protein (S) contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor, angiotensin converting enzyme-2 (ACE2). It is found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2 receptor leading to endocytosis into the host cells of the deep lung and viral replication. Infection with the SARS-CoV-2 initiates an immune response, which includes the production of antibodies in the blood. The secreted antibodies provide protection against future infections from viruses, because they remain in the circulatory system for months to years after infection and will bind quickly and strongly to the pathogen to block cellular infiltration and replication. These antibodies are named neutralizing antibodies. This kit can detect circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain of the viral spike glycoprotein (RBD) with the ACE2 cell surface receptor. The detection is not limited by both species and antibody isotypes.
Background/Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, or 2019-nCoV) is an enveloped non-segmented positive-sense RNA virus. It is the cause of coronavirus disease 2019 (COVID-19), which is contagious in humans. SARS-CoV-2 has several structural proteins including spike (S), envelope (E),membrane (M) and nucleocapsid (N). The spike protein (S) contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor, angiotensin converting enzyme-2 (ACE2). It is found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2 receptor leading to endocytosis into the host cells of the deep lung and viral replication. Infection with the SARS-CoV-2 initiates an immune response, which includes the production of antibodies in the blood. The secreted antibodies provide protection against future infections from viruses, because they remain in the circulatory system for months to years after infection and will bind quickly and strongly to the pathogen to block cellular infiltration and replication. These antibodies are named neutralizing antibodies. This kit can detect circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain of the viral spike glycoprotein (RBD) with the ACE2 cell surface receptor. The detection is not limited by both species and antibody isotypes.
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Product Notes
The COVID-19 (Catalog #AAA355919) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 Neutralizing Coronavirus, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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