Fatty Acid Amide Hydrolase Control Peptide | FAAH control peptide
Fatty Acid Amide Hydrolase (FAAH) (Control Peptide)
Purified
Purified
Dilution: ELISA: 50-100ng of control peptide/well
A 17 amino acid peptide sequence near the N-terminus of human FAAH. Peptide is 100% conserved in rat, 94% in mouse and porcine and 58% in chicken. No significant homology exists with other hydrolases.
Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis. Cannabis affect cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoid, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2. Neurons and astrocytes have been found to re-uptake and hydrolyse anandamide rapidly, resulting in the formation of arachidonic acid and ethanolamine. The uptake mechanism has been shown to be mediated by a saturable, selective, temperature-dependent and Na+-independent transporter. Anandamide hydrolysis is catalyzed by a membrane-bound amidohydrolase (called anandamide amidohydrolase or fatty acid amide hydrolase, FAAH). FAAH (rat/mouse/human 579 aa; chromosome 1p34-p35; mol wt ~67 kD) sequence analyses suggest a single predicted transmembrane domain at the extreme N-terminus of the enzyme. Distribution of FAAH parallels CB1 in rat brain suggesting that FAAH participates in cannabinoid signalling mechanisms. The sn-2-Arachidonylglycerol (2-AG), initially isolated from intestine, appears to be the second endogenous CB ligand (CB1 Ki 472 nM; CB2 Ki 1400 nM). 2-AG concentration in the brain is 170 times greater than anandamide. FAAH hydrolyzes 2-AG at a rate four times faster than that for anandamide hydrolysis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. Ref.8
Catalytic activity: Anandamide + H2O = arachidonic acid + ethanolamine.Oleamide + H2O = oleic acid + NH3.
Enzyme regulation: Inhibited by O-aryl carbamates and alpha-keto heterocytes. Ref.8
Subunit structure: Homodimer
By similarity.
Subcellular location: Endomembrane system; Single-pass membrane protein. Cytoplasm › cytoskeleton. Note: Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. Ref.8
Tissue specificity: Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. Ref.8
Polymorphism: Variant Thr-129 seems to be strongly associated with illegal drug use and dependence. This variant displays normal catalytic properties but an enhanced sensitivity to proteolytic degradation.
Sequence similarities: Belongs to the amidase family.
Research Articles on FAAH
Similar Products
Product Notes
The FAAH faah (Catalog #AAA653955) is a Control Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fatty Acid Amide Hydrolase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA, Antibody Blocking. Dilution: ELISA: 50-100ng of control peptide/well. Researchers should empirically determine the suitability of the FAAH faah for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fatty Acid Amide Hydrolase, Control Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
