Typical Testing Data/Standard Curve
Human Fatty acid amide hydrolase 1 (FAAH) ELISA Kit | FAAH elisa kit
Human Fatty acid amide hydrolase 1 (FAAH) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||FAAH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FAAH1 antibody and an FAAH1-HRP conjugate. The assay sample and buffer are incubated together with FAAH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAAH1 concentration since FAAH1 from samples and FAAH1-HRP conjugate compete for the anti-FAAH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FAAH1 from the sample, fewer sites are left to bind FAAH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAAH1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]
Uniprot Description
FAAH: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. Homodimer. Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. Inhibited by O-aryl carbamates and alpha-keto heterocytes. Belongs to the amidase family.
Protein type: EC 3.5.1.99; Hydrolase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p35-p34
Cellular Component: cytoskeleton; cytoplasm; integral to membrane; endomembrane system
Molecular Function: fatty acid amide hydrolase activity; carbon-nitrogen ligase activity, with glutamine as amido-N-donor; acylglycerol lipase activity
Biological Process: fatty acid catabolic process
Disease: Polysubstance Abuse, Susceptibility To
Research Articles on FAAH
Similar Products
Product Notes
The Human FAAH faah (Catalog #AAA7231327) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231327 ELISA Kit recognizes Human FAAH. It is sometimes possible for the material contained within the vial of "Fatty acid amide hydrolase 1 (FAAH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
