Typical Testing Data/Standard Curve (for reference only)
Human Fatty-acid amide hydrolase ELISA Kit | FAAH elisa kit
Human FAAH (Fatty-acid amide hydrolase) ELISA kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated withan antibody specific to Human F8. Standards or samples are added to the micro ELISA plate wells and combined with thespecific antibody. Then a biotinylated detection antibody specific for Human F8 and Avidin-Horseradish Peroxidase(HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. Thesubstrate solution is added to each well. Only those wells that contain Human F8, biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stopsolution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm +/- 2 nm. The OD value is proportional to the concentration of Human F8. You can calculate the concentration ofHuman F8 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]
Uniprot Description
FAAH: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. Homodimer. Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. Inhibited by O-aryl carbamates and alpha-keto heterocytes. Belongs to the amidase family.
Protein type: EC 3.5.1.99; Hydrolase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p35-p34
Cellular Component: cytoskeleton; endoplasmic reticulum membrane; integral to membrane
Molecular Function: acylglycerol lipase activity; carbon-nitrogen ligase activity, with glutamine as amido-N-donor; fatty acid amide hydrolase activity; protein binding
Biological Process: arachidonic acid metabolic process; fatty acid catabolic process
Disease: Polysubstance Abuse, Susceptibility To
Research Articles on FAAH
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Product Notes
The Human FAAH faah (Catalog #AAA2533409) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533409 ELISA Kit recognizes Human FAAH. It is sometimes possible for the material contained within the vial of "Fatty-acid amide hydrolase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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