Collagen Type VI Alpha 1 (COL6a1) Recombinant Protein | COL6a1 recombinant protein

Recombinant Collagen Type VI Alpha 1 (COL6a1)

Cat. Num. AAA2010589

• Gene Names: Col6a1; Col6a-1; AI747156
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Collagen Type VI Alpha 1 (COL6a1); Recombinant Collagen Type VI Alpha 1 (COL6a1); COL6a1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-SIGLQNF EIAKDFIIKV IDRLSKDELV KFEPGQSHAG VVQYSHNQMQ EHVDMRSPNV RNAQDFKEAV KKLQWMAGGT FTGEALQYTR DRLLPPTQNN RIALVITDGR SDTQRDTTPL SVLCGADIQV VSVGIKDVFG FVAGSDQLNV ISCQGLSQGR PGISLVKENY AELLDDGFLK
• Sequence Length: 1025

• Applicable Applications for COL6a1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser624~Lys800 (Accession # Q04857) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 6753484
• NCBI GeneID: 12833
• NCBI Accession #: NP_034063.1
• NCBI GenBank Nucleotide #: NM_009933.4
• UniProt Accession #: Q04857
• Molecular Weight: 21.1kDa
• NCBI Official Full Name: collagen alpha-1(VI) chain
• NCBI Official Synonym Full Names: collagen, type VI, alpha 1
• NCBI Official Symbol: Col6a1
• NCBI Official Synonym Symbols: Col6a-1; AI747156
• NCBI Protein Information: collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; procollagen, type VI, alpha 1
• UniProt Protein Name: Collagen alpha-1(VI) chain
• Protein Family: Collagen
• UniProt Gene Name: Col6a1
• UniProt Entry Name: CO6A1_MOUSE

Uniprot Description

COL6A1: Collagen VI acts as a cell-binding protein. Defects in COL6A1 are a cause of Bethlem myopathy (BM). BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles. Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD); also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. Belongs to the type VI collagen family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; protein complex; membrane; lysosomal membrane; extracellular region; sarcolemma

Molecular Function: platelet-derived growth factor binding

Biological Process: osteoblast differentiation; cell adhesion