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Mouse collagen, type V, alpha 1 ELISA Kit | COL5A1 elisa kit

Mouse Collagen alpha-1 (V) chain, COL5A1 ELISA Kit

Gene Names
Col5a1; AI413331
Reactivity
Mouse
Synonyms
collagen; type V; alpha 1; Mouse Collagen alpha-1 (V) chain; COL5A1 ELISA Kit; Mouse Collagen alpha-1 (V) chain (COL5A1) ELISA kit; RP11-263F14.1; alpha 1 type V collagen; COL5A1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of human COL5A1. No significant cross-reactivity or interference between human COL5A1 and analogues was observed.
Sequence Length
1838
Samples
Serum, Plasma And Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for COL5A1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
183,677 Da
NCBI Official Full Name
collagen alpha-1(V) chain
NCBI Official Synonym Full Names
collagen, type V, alpha 1
NCBI Official Symbol
Col5a1
NCBI Official Synonym Symbols
AI413331
NCBI Protein Information
collagen alpha-1(V) chain; collagen alpha-1(V) chain; alpha1(V) collagen; pro-alpha1(V) collagen; procollagen type V alpha 1; procollagen, type V, alpha 1
UniProt Protein Name
Collagen alpha-1(V) chain
Protein Family
UniProt Gene Name
Col5a1
UniProt Entry Name
CO5A1_MOUSE

Uniprot Description

COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.

Protein type: Secreted; Extracellular matrix; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; collagen type V; extracellular region; basement membrane

Molecular Function: heparin binding; protein binding; proteoglycan binding; extracellular matrix structural constituent; platelet-derived growth factor binding

Biological Process: heart morphogenesis; skin development; blood vessel development; cell migration; integrin biosynthetic process; collagen fibril organization; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; eye morphogenesis; wound healing, spreading of epidermal cells

Research Articles on COL5A1

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Product Notes

The Mouse COL5A1 col5a1 (Catalog #AAA932248) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA932248 ELISA Kit recognizes Mouse COL5A1. It is sometimes possible for the material contained within the vial of "collagen, type V, alpha 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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