Procollagen II C-Terminal Propeptide (PIICP) Recombinant Protein | PIICP recombinant protein

Recombinant Procollagen II C-Terminal Propeptide (PIICP)

Cat. Num. AAA2105319

• Gene Names: Col2a1; Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100413; Rgsc413; Rgsc856
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Procollagen II C-Terminal Propeptide (PIICP); Recombinant Procollagen II C-Terminal Propeptide (PIICP); P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide; PIICP recombinant protein

• Host: Host: E Coli; Source: Prokaryotic Expression
• Purity/Purification: > 95%
• Form/Format: Freeze-Dried Powder; PBS, pH7.4, Containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Asp1242~Leu1487
• Sequence Length: 1487

• Applicable Applications for PIICP recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Species: Mouse
• Tag: N-terminal His-Tag
• Isoelectric Point: 6.5
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Subcellular Location: Secreted
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for PIICP recombinant protein

Infection immunity; Bone metabolism; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 125987808
• NCBI GeneID: 12824
• UniProt Accession #: P28481
• Molecular Weight: Predicted MW: 31.2kDa; Accurate MW: 35kDa
• NCBI Official Full Name: Collagen alpha-1(II) chain
• NCBI Official Synonym Full Names: collagen, type II, alpha 1
• NCBI Official Symbol: Col2a1
• NCBI Official Synonym Symbols: Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100413; Rgsc413; Rgsc856
• NCBI Protein Information: collagen alpha-1(II) chain
• UniProt Protein Name: Collagen alpha-1(II) chain
• UniProt Gene Name: Col2a1
• UniProt Entry Name: CO2A1_MOUSE

NCBI Description

This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]

Uniprot Description

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; collagen; collagen type II; cytoplasm; extracellular region; basement membrane

Molecular Function: identical protein binding; extracellular matrix structural constituent; metal ion binding; platelet-derived growth factor binding

Biological Process: heart morphogenesis; proteoglycan metabolic process; inner ear morphogenesis; collagen fibril organization; palate development; limb morphogenesis; sensory perception of sound; visual perception; skeletal morphogenesis; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; inner ear development; skeletal development; cartilage condensation; endochondral ossification