Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CNTN2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CNTN2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CNTN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CNTN2. You can calculate the concentration of CNTN2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention. [provided by RefSeq, Jul 2008]
Uniprot Description
CNTN2: May play a role in the initial growth and guidance of axons. May be involved in cell adhesion. Belongs to the immunoglobulin superfamily. Contactin family.
Protein type: Membrane protein, GPI anchor; Cell adhesion
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: voltage-gated potassium channel complex; cell surface; cell soma; integral to plasma membrane; plasma membrane; synapse; myelin sheath
Molecular Function: identical protein binding; protein self-association; glycoprotein binding; carbohydrate binding
Biological Process: axon guidance; negative regulation of neuron differentiation; cerebral cortex GABAergic interneuron migration; receptor internalization; myelination in the central nervous system; clustering of voltage-gated potassium channels; positive regulation of adenosine receptor signaling pathway; regulation of axon diameter; axonal fasciculation; regulation of astrocyte differentiation; learning; microtubule cytoskeleton organization and biogenesis; cell adhesion; adult walking behavior; regulation of neuronal synaptic plasticity
Disease: Epilepsy, Familial Adult Myoclonic, 5
Research Articles on CNTN2
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Product Notes
The Mouse CNTN2 cntn2 (Catalog #AAA2506047) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506047 ELISA Kit recognizes Mouse CNTN2. It is sometimes possible for the material contained within the vial of "CNTN2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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