Human Calponin-2 ELISA Kit | CNN2 elisa kit

Human Calponin-2 ELISA Kit

Cat. Num. AAA762582

• Reactivity: Human
• Synonyms: Calponin-2; Human Calponin-2 ELISA Kit; CNN2/Calponin-2/Calponin H2; smooth muscle/Neutral calponin; CNN2 elisa kit

• Reactivity: Human
• Sequence Length: 330

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CNN2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CNN2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CNN2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CNN2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CNN2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 746576376
• NCBI GeneID: 1265
• NCBI Accession #: NP_001290430.1
• NCBI GenBank Nucleotide #: NM_001303501.1
• Molecular Weight: 29,500 Da
• NCBI Official Full Name: calponin-2 isoform d
• NCBI Official Synonym Full Names: calponin 2
• NCBI Official Symbol: CNN2
• NCBI Protein Information: calponin-2
• UniProt Protein Name: Calponin-2
• Protein Family: Calponin
• UniProt Gene Name: CNN2
• UniProt Entry Name: CNN2_HUMAN

NCBI Description

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

Uniprot Description

calponin 2: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoskeleton; focal adhesion; intercellular junction; membrane; stress fiber

Molecular Function: actin binding; calmodulin binding

Biological Process: actomyosin structure organization and biogenesis; cytoskeleton organization and biogenesis; regulation of actin filament-based process