Mouse Calponin-2 (CNN2) ELISA Kit | CNN2 elisa kit

Mouse Calponin-2 (CNN2) ELISA Kit

Cat. Num. AAA7226991

• Reactivity: Mouse
• Synonyms: Calponin-2 (CNN2); Mouse Calponin-2 (CNN2) ELISA Kit; CNN2 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CNN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CNN2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNN2 elisa kit

Principle of the assay: CNN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNN2 antibody and an CNN2-HRP conjugate. The assay sample and buffer are incubated together with CNN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNN2 concentration since CNN2 from samples and CNN2-HRP conjugate compete for the anti-CNN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNN2 from the sample, fewer sites are left to bind CNN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNN2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CNN2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4758018
• NCBI GeneID: 1265
• NCBI Accession #: NP_004359.1
• NCBI GenBank Nucleotide #: NM_004368.2
• Molecular Weight: 29,500 Da
• NCBI Official Full Name: calponin-2 isoform a
• NCBI Official Synonym Full Names: calponin 2
• NCBI Official Symbol: CNN2
• NCBI Protein Information: calponin-2; neutral calponin; calponin H2, smooth muscle
• UniProt Protein Name: Calponin-2
• Protein Family: Calponin
• UniProt Gene Name: CNN2
• UniProt Entry Name: CNN2_HUMAN

NCBI Description

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

calponin 2: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: focal adhesion; cytoskeleton; membrane; stress fiber; intercellular junction

Molecular Function: calmodulin binding; actin binding

Biological Process: regulation of actin filament-based process; negative regulation of phagocytosis; wound healing; actomyosin structure organization and biogenesis; cytoskeleton organization and biogenesis; hemopoiesis; negative regulation of cell migration; regulation of cell proliferation