Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1) Recombinant Protein | clp1 recombinant protein

Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1)

Cat. Num. AAA1408275

• Gene Names: clp1.L; clp1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1); Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1); clp1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-439aa; Full Length
• Sequence: MSSEDLSAPGPSAPDSSWSSTGPSTKFELERETELRLEVEGTDPVRVELVSGLAEVFGTELTRNKKYTFPPGSRAAIFTWHGCTVQLWGSPDMAYVSRDTPMLLYLNTQVGLEQMRVQAEREGERGPRVLVAGPSDVGKSTLCRLLLNYAVRRGRRPTLVELDVGQGSVSVPGTMGALCVERPADVEEGFSAQAPLVYHFGSTTPGTNIKLYNKLTSRLAHVFNLRCDSNRRASVSGCLINTCGWVKGSGYQALIHAASAFEVDVVLVLDQERLYNDLLRDLPHFVRTLLLPKSGGASERSKECRRESRDQRVREYFYGPRGSLYPHAFEIKFSEVRVYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMAHHLLSVVPLDGGSAEEGIEERSVAGFIVITGVDTERQTLTLLSPAPRPLPKCVLLIMDIRFMDLK

• Species: Xenopus laevis (African clawed frog)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 148234409
• NCBI GeneID: 431824
• NCBI Accession #: NP_001084787.1
• NCBI GenBank Nucleotide #: NM_001091318.1
• UniProt Accession #: Q6NS21
• Molecular Weight: 48,336 Da
• NCBI Official Full Name: polyribonucleotide 5'-hydroxyl-kinase Clp1
• NCBI Official Synonym Full Names: cleavage and polyadenylation factor I subunit 1 L homeolog
• NCBI Official Symbol: clp1.L
• NCBI Official Synonym Symbols: clp1
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• Protein Family: Polyribonucleotide 5'-hydroxyl-kinase
• UniProt Gene Name: clp1

Uniprot Description

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by dicer1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing ().