Typical Testing Data/Standard Curve (for reference only)
Interferon Alpha 14 (IFNa14) CLIA Kit | IFNa14 clia kit
CLIA Kit for Interferon Alpha 14 (IFNa14)
Gene Names
IFNA14; LEIF2H; IFN-alphaH
Synonyms
Interferon Alpha 14 (IFNa14); CLIA Kit for Interferon Alpha 14 (IFNa14); IFNa14 clia kit
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 14 (IFNa14)
No significant cross-reactivity or interference between Interferon Alpha 14 (IFNa14) and analogues was observed.
No significant cross-reactivity or interference between Interferon Alpha 14 (IFNa14) and analogues was observed.
Sequence Length
189
Assay Type
Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
1.372-1000pg/mL The standard curve concentrations used for the ELISA's were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.346pg/mL, 4.115pg/mL, 1.372pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 6.9pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 14 (IFNa14) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 14 (IFNa14) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 14 (IFNa14) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Organism Species
Homo sapiens (Human)
Assay Length
4.5 hours
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IFNa14 clia kit
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 14 (IFNa14) level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,063 Da
NCBI Official Full Name
interferon alpha-14
NCBI Official Synonym Full Names
interferon, alpha 14
NCBI Official Symbol
IFNA14
NCBI Official Synonym Symbols
LEIF2H; IFN-alphaH
NCBI Protein Information
interferon alpha-14; leIF H; IFN-alpha-14; interferon alpha-H; interferon lambda-2-H
UniProt Protein Name
Interferon alpha-14
Protein Family
UniProt Gene Name
IFNA14
UniProt Synonym Gene Names
IFN-alpha-14; LeIF H
UniProt Entry Name
IFN14_HUMAN
Uniprot Description
IFNA14: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.
Protein type: Secreted, signal peptide; Membrane protein, integral; Secreted
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space; extracellular region
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
Research Articles on IFNa14
1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
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Product Notes
The IFNa14 ifna14 (Catalog #AAA2001232) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interferon Alpha 14 (IFNa14), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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