Dectin-1 Recombinant Protein | CLEC7A recombinant protein

Recombinant Human Dectin-1 Protein (N-Fc)

Cat. Num. AAA2557315

• Gene Names: CLEC7A; BGR; CD369; CANDF4; SCARE2; DECTIN1; CLECSF12
• Purity: >85% as determined by reducing SDS-PAGE.
• Synonyms: Dectin-1; Recombinant Human Dectin-1 Protein (N-Fc); Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; Dectin1; DECTIN1CANDF4; CLEC7A recombinant protein

• Host: Human Cells
• Purity/Purification: >85% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Thr66-Met247
• Sequence Length: 201

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: N-Fc
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CLEC7A recombinant protein

Protein Construction: Recombinant Human Dectin-1 is produced by our mammalian expression system and the target gene encoding Thr66-Met247 is expressed with a Fc tag at the N-terminus.

Background: Dectin-1 was recently identified as the most important receptor for beta-glucan. It is a type II transmembrane protein which binds beta-1,3 and beta-1,6 glucans, and is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. Recognition of beta-glucan by dectin-1 triggers effective immune response, including phagocytosis and proinflammatory factor production, to eliminate infecting fungi, which especially benefits immunocompromised patients against opportunistic fungal infection.

NCBI and Uniprot Product Information

• NCBI GI #: 13384604
• NCBI GeneID: 64581
• NCBI Accession #: NP_072092.2
• NCBI GenBank Nucleotide #: NP_072092.2
• UniProt Accession #: Q9BXN2
• Molecular Weight: Molecular Mass: 46.9kDa; Actual Protein Molecular Mass: 50-65kDa
• NCBI Official Full Name: C-type lectin domain family 7 member A isoform b
• NCBI Official Synonym Full Names: C-type lectin domain containing 7A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CD369; CANDF4; SCARE2; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A
• UniProt Protein Name: C-type lectin domain family 7 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; plasma membrane; integral to membrane

Molecular Function: metal ion binding; carbohydrate binding; MHC protein binding; pattern recognition receptor activity

Biological Process: T cell activation; innate immune response; carbohydrate mediated signaling; phagocytosis, recognition; cell recognition; pattern recognition receptor signaling pathway; defense response to protozoan; inflammatory response

Disease: Candidiasis, Familial, 4; Aspergillosis, Susceptibility To