Rabbit BLK Polyclonal Antibody | anti-BLK antibody

Anti-BLK (pY389) Phospho Antibody

Cat. Num. AAA8303931

• Gene Names: BLK; MODY11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by immunogen affinity chromatography.
• Synonyms: BLK; Polyclonal Antibody; Anti-BLK (pY389) Phospho Antibody; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk; anti-BLK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of BLK (pY389) protein.
• Purity/Purification: Purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
• Sequence Length: 505

• Applicable Applications for anti-BLK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BLK.
• Preparation and Storage: Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of BLK (pY389) expression in HEK293T (A), PANC1 (B), CT26 (C), rat spleen (D) whole cell lysates.)

Related Product Information for anti-BLK antibody

Rabbit polyclonal antibody to BLK (pY389)

NCBI and Uniprot Product Information

• NCBI GI #: 33469982
• NCBI GeneID: 640
• NCBI Accession #: NP_001706.2
• NCBI GenBank Nucleotide #: NM_001715.2
• UniProt Accession #: P51451; Q16291; Q96IN1
• Molecular Weight: 57,706 Da
• NCBI Official Full Name: tyrosine-protein kinase Blk isoform 1
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.