Typical Testing Data/Standard Curve (for reference only)
Mouse CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase 1 (ST3GAL1) ELISA Kit | ST3GAL1 elisa kit
Mouse CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase 1 (ST3GAL1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ST3GAL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ST3GAL1 antibody and an ST3GAL1-HRP conjugate. The assay sample and buffer are incubated together with ST3GAL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ST3GAL1 concentration since ST3GAL1 from samples and ST3GAL1-HRP conjugate compete for the anti-ST3GAL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ST3GAL1 from the sample, fewer sites are left to bind ST3GAL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST3GAL1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq, Jul 2008]
Uniprot Description
ST3GAL1: It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O- linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values. Belongs to the glycosyltransferase 29 family.
Protein type: Glycan Metabolism - O-glycan biosynthesis; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; EC 2.4.99.4
Chromosomal Location of Human Ortholog: 8q24.22
Cellular Component: Golgi membrane; membrane; integral to Golgi membrane
Molecular Function: beta-galactoside alpha-2,3-sialyltransferase activity
Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; glycosaminoglycan metabolic process; dolichol-linked oligosaccharide biosynthetic process; O-glycan processing; protein amino acid N-linked glycosylation; protein modification process; pathogenesis; post-translational protein modification; protein amino acid phosphorylation; cellular protein metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; protein amino acid N-linked glycosylation via asparagine; N-acetylneuraminate metabolic process
Research Articles on ST3GAL1
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Product Notes
The Mouse ST3GAL1 st3gal1 (Catalog #AAA7235806) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235806 ELISA Kit recognizes Mouse ST3GAL1. It is sometimes possible for the material contained within the vial of "CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase 1 (ST3GAL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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