DC-SIGNR/CD299 recombinant protein

Recombinant Human DC-SIGNR/CD299 (N-8His-DYKDDDDK)

Cat. Num. AAA2569173

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: DC-SIGNR/CD299; Recombinant Human DC-SIGNR/CD299 (N-8His-DYKDDDDK); CD209L; CD209L1; CD299; CLEC4M; C-type lectin domain family 4; member M; DC-SIGN2; DCSIGNR; DC-SIGNR; HP10347; LSIGN; L-SIGN; C-type Lectin Domain Family 4 Member M; DC-SIGNR/CD299 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Ser73-Glu376

• Species: Human
• Tag: N-8His-DYKDDDDK
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human C-type Lectin Domain Family 4 Member M is produced by our Mammalian expression system and the target gene encoding Ser73-Glu376 is expressed with a 8His, DYKDDDDK tag at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

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Related Product Information for DC-SIGNR/CD299 recombinant protein

CD299 is also known as DC-SIGNR and CLEC4M, is a type II integral membrane protein. DC-SIGNR exists as a homotetramer, and the tandem repeat domain, also called neck domain, mediates oligermerization. Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms. DC-SIGNR is ragarded as a pathogen-recognition receptor involved in peripheral immune surveillance in liver, and probably mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. DC-SIGNR appears to selectively recognize and bind many viral surface glycoproteins containing high mannose N-linked oligosaccharides in a calcium-dependent manner, including HIV-1 gp12, HIV-2 gp12, SIV gp12, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S, as well as the cellular adhesion protein ICAM3. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation.

NCBI and Uniprot Product Information

• UniProt Accession #: Q9H2X3
• Molecular Weight: Mol. Mass: 37kDa; AP Mol. Mass: 35-40kDa
• UniProt Protein Name: C-type lectin domain family 4 member M
• UniProt Gene Name: CLEC4M
• UniProt Synonym Gene Names: CD209L; CD209L1; CD299; DC-SIGNR; DC-SIGN2; L-SIGN
• UniProt Entry Name: CLC4M_HUMAN

Uniprot Description

CLEC4M: Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: integral to plasma membrane; membrane

Molecular Function: calcium-dependent protein binding; viral receptor activity; virion binding

Biological Process: cell-cell recognition; regulation of blood coagulation; regulation of gene expression; virion attachment to host cell surface receptor; virus-host interaction