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Typical Testing Data/Standard Curve (for reference only)

Rat Galanin (GAL) CLIA Kit | GAL clia kit

Rat Galanin (GAL) CLIA Kit

Gene Names
Gal; Galn
Reactivity
Rat
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Galanin (GAL); Rat Galanin (GAL) CLIA Kit; Galanin; GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide; GAL clia kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Galanin (GAL).
No significant cross-reactivity or interference between Galanin (GAL) and analogues was observed.
Sequence Length
124
Applicable Applications for GAL clia kit
Chemiluminescence Immunoassay (CLIA)
Assay Type
Competitive Inhibition
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.81-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 3.15pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Galanin (GAL). A competitive inhibition reaction is launched between biotin labeled Galanin (GAL) and unlabeled Galanin (GAL) (Standards or samples) with the pre-coated antibody specific to Galanin (GAL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Galanin (GAL) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Galanin (GAL) level in the sample or standard.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
galanin peptides preproprotein
NCBI Official Synonym Full Names
galanin and GMAP prepropeptide
NCBI Official Symbol
Gal
NCBI Official Synonym Symbols
Galn
NCBI Protein Information
galanin peptides
UniProt Protein Name
Galanin peptides
Protein Family
UniProt Gene Name
Gal
UniProt Synonym Gene Names
Galn; GMAP

NCBI Description

binds G-protein coupled receptors GalR1, GalR2, and GalR3; involved in negative regulation of immature thymocyte proliferation; plays a role in regulation of the immune system [RGD, Feb 2006]

Uniprot Description

Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion.

Research Articles on GAL

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Product Notes

The Rat GAL gal (Catalog #AAA2707950) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707950 CLIA Kit recognizes Rat GAL. AAA Biotech's Galanin (GAL) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the GAL gal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galanin (GAL), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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