Human cytokeratin 18 ELISA Kit | CK-18 elisa kit
Human cytokeratin 18 (CK-18) ELISA Kit
Principle of the Assay: This assay employs the sandwich enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to CK-18. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for CK-18 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain CK-18, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CK-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.
Protein type: Cytoskeletal; Nucleolus
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cell periphery; cell-cell adherens junction; cytoplasm; cytosol; extracellular exosome; intermediate filament; keratin filament; microtubule organizing center; nucleolus; perinuclear region of cytoplasm
Molecular Function: cadherin binding involved in cell-cell adhesion; protein binding; RNA binding; structural molecule activity
Biological Process: anatomical structure morphogenesis; cell cycle; cornification; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; keratinization; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; viral process
Disease: Cirrhosis, Familial
Research Articles on CK-18
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Product Notes
The Human CK-18 krt18 (Catalog #AAA281568) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281568 ELISA Kit recognizes Human CK-18. It is sometimes possible for the material contained within the vial of "cytokeratin 18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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