Frizzled 5 Cell Lysate | FZD5 cell lysate
Mouse Frizzled 5 CHO Stable Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Samples are stable for up to twelve months from date of receipt.
This Mouse Frizzled 5 overexpression lysate was created in CHO Stable Cells and intented for use as a Western blot (WB) positive control. Purification of Frizzled 5 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
Uniprot Description
FZD5: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway. Interacts with GOPC. Belongs to the G-protein coupled receptor Fz/Smo family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, Fz/Smo family; Receptor, GPCR
Cellular Component: Golgi apparatus; cell surface; tight junction; membrane; perinuclear region of cytoplasm; early endosome; integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; signal transducer activity; protein binding; Wnt receptor activity; transmembrane receptor activity; ubiquitin protein ligase binding; protein kinase binding
Biological Process: positive regulation of JNK activity; post-embryonic camera-type eye development; Wnt receptor signaling pathway; multicellular organismal development; embryonic axis specification; cell maturation; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; signal transduction; embryonic camera-type eye development; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; vasculature development; determination of anterior/posterior axis, embryo; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; cell differentiation; Spemann organizer formation
Research Articles on FZD5
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Product Notes
The FZD5 fzd5 (Catalog #AAA8116863) is a Cell Lysate produced from CHO Stable Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 382aa. AAA Biotech's Frizzled 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the FZD5 fzd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro16 7. It is sometimes possible for the material contained within the vial of "Frizzled 5, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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