R-Spondin-1 Active Protein | RSPO1 active protein

Recombinant Human R-Spondin-1

Cat. Num. AAA141446

• Gene Names: Rspo1; Rspondin; R-spondin
• Purity: >95.0% as determined by SDS-PAGE.
• Synonyms: R-Spondin-1; Recombinant Human R-Spondin-1; R-spondin-1; Cristin-3; mCristin-3; Roof plate-specific spondin-1; Rspo1; RSPO; R-spondin 1; R-spondin; Rspondin; CRISTIN3; RSPO1 Human; R-Spondin-1 Human Recombinant; RSPO1 active protein

• Host: CHO Cells
• Purity/Purification: >95.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered white lyophilized (freeze-dried) powder.; The protein was lyophilized from a sterile (0.2um) filtered solution containing PBS.
• Sequence: Amino acid: RISAEGSQAC AKGCELCSEVngCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA.
• Sequence Length: 265

• Solubility: It is recommended to reconstitute the lyophilized RSPO1in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50, as calculated by the Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a is 24.7ng/ml corresponding to a specific activity of 4.0 x 10^4 units/mg.
• Preparation and Storage: Lyophilized RSPO1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C.; Upon reconstitution RSPO1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.; Please prevent freeze-thaw cycles.

Related Product Information for RSPO1 active protein

Introduction: R-Spondin-1 (Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Rspo1 induces the onset of crypt cell proliferation and increases intestinal epithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway.

Description: R-Spondin-1 Human Recombinant produced in CHO cells is a glycosylated monomer chain containing 233 amino acids and having a total molecular mass of 25.6kDa.RSPO1 is purified by proprietary chromatographic techniques.

Product Categories/Family for RSPO1 active protein

RECOMBINANT & NATURAL PROTEINS

NCBI and Uniprot Product Information

• NCBI GI #: 227452351
• NCBI GeneID: 192199
• NCBI Accession #: NP_619624.2
• NCBI GenBank Nucleotide #: NP_619624.2
• UniProt Accession #: Q9Z132
• NCBI Official Full Name: R-spondin-1
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: Rspo1
• NCBI Official Synonym Symbols: Rspondin; R-spondin
• NCBI Protein Information: R-spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: Rspo1
• UniProt Synonym Gene Names: Cristin-3; mCristin-3

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4|4 D2.2

Cellular Component: extracellular space; nucleus

Molecular Function: G-protein-coupled receptor binding; heparin binding; protein binding; receptor binding

Biological Process: male meiosis; positive regulation of protein amino acid phosphorylation; positive regulation of Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin