CD27 protein

CD27-muIg-Purified (with Antibiotics)

Cat. Num. AAA666807

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Purified (with Antibiotics)
• Synonyms: CD27; CD27-muIg-Purified (with Antibiotics); Human CD27-muIg Fusion Protein; CD27 protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for CD27 protein: Flow Cytometry (FC/FACS), ELISA (EIA)
• Molecular Structure: A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker: (1)kpqapelrgs(10) A CD70-reactive n-terminal section of the mature extracellular domain of human CD27: (11)kscperhywaqgklccqmcepgtflvkdcdqhrkaaqcdpcipgvsfspdhhtrphcescrhcnsgllvrnctitanaecacrngwqcrdkectecdplpnps (113) linker (114)gt(115) murine IgG2a Fc + hinge regions: (116) eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk (348) The molecule is dimeric with a predicted monomeric non glycosylated molecular weight of 39.3 kd.
• Transfectant Cell Line: CHO
• Performance: Five x 105 cultured human Raji cells were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of CD27-muIg 20 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.4 log10 fluorescent units when compared to a muIg-Fc negative control at a similar concentration. Binding was blocked when reagent was pre incubated with a 2.5-fold excess (mg/mg) of recombinant soluble CD70-muCD8.
• Production: Human CD27-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin sulfate (as a preservative).
• Group Header: CD27-muIg
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 6 months after shipping date when stored as recommended.

Testing Data

Related Product Information for CD27 protein

Information: Human CD27 is a lymphocyte specific member of the tumor necrosis factor receptor family (TNFRSF7) and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily(TNFSF7). The CD27-CD70 interaction plays an important role in T cell activation. Recombinant soluble CD27-muIg binds to cell surface CD70 on Raji cells in FACS, and is reactive with recombinant CD70-muCD8, and anti-CD27 mAb clone M-T271.

References

1) Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 350-352. 2) K. Agematsu, et al.(1994) J Immunol 153(4): 1421-1429. 3) R.Q. Hintzen, et al, (1994) Immunol Today 15: 307-311. 4) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 356-360, 435-437. 5) K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
• UniProt Protein Name: CD27 antigen
• Protein Family: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7
• UniProt Entry Name: CD27_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Membrane protein, integral; Receptor, cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity

Biological Process: release of cytoplasmic sequestered NF-kappaB; cell surface receptor linked signal transduction; response to ethanol; positive regulation of JNK cascade; positive regulation of B cell differentiation; negative regulation of caspase activity; immunoglobulin mediated immune response; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome 2