CD154 CD8a, Fusion Protein

Applications

ELISA, Western Blot, Flow Cytometry, Functional Assay

Purity

Affinity Purified
Purified by affinity chromatography.

Synonyms

CD154 CD8a; Fusion Protein; Fusion Protein protein

Ordering
Cat. Num. AAA634458 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Host

CHO cells

Purity/Purification

Affinity Purified
Purified by affinity chromatography.

Form/Format

Supplied as a liquid in 50mM Tris 150mM NaCl 1mM Spermidine pH 8.0, 0.2% BSA, 0.5 mg/ml Gentamicin Sulfate.

Applicable Applications for CD154 CD8a, Fusion Protein protein

ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Application Notes

Suitable for use in Flow Cytometry, ELISA and Western Blot.

Molecular Structure

A soluble fusion protein consisting of the extracellular domain (213aa) of human CD154 fused to the extracellular domain (167aa) of murine CD8 alpha.

Functional Application

Human CD154-muCD8 binds to cell surface expressed human CD40 and this binding is blocked by anti-human CD154 monoclonal antibody.
Human CD154 (CD40 Ligand) muCD8 Fusion Protein*

Transfectant Cell Line

CHO

Preparation and Storage

May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CD154 CD8a, Fusion Protein protein

Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). CD154 has been shown to be hyperexpressed by B and T cells in SLE patients (4). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6).

Product Categories/Family for CD154 CD8a, Fusion Protein protein

Molecular Biology; MB-CD Markers

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Product Notes

The CD154 CD8a, Fusion Protein (Catalog #AAA634458) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD154 CD8a, Fusion Protein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, ELISA and Western Blot. Researchers should empirically determine the suitability of the CD154 CD8a, Fusion Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD154 CD8a, Fusion Protein, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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CD154 CD8a, Fusion Protein