Rabbit anti-Human, Mouse MOCS1 Polyclonal Antibody | anti-MOCS1 antibody

MOCS1, CT (MOCS1, Molybdenum cofactor biosynthesis protein 1, Cell migration-inducing gene 11 protein, Molybdenum cofactor synthesis-step 1 protein A-B, Molybdenum cofactor biosynthesis protein A, Molybdenum cofactor biosynthesis protein C) (Azide free) (

Cat. Num. AAA6321596

• Gene Names: MOCS1; MIG11; MOCOD; MOCS1A; MOCS1B
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MOCS1; Polyclonal Antibody; CT (MOCS1; Molybdenum cofactor biosynthesis protein 1; Cell migration-inducing gene 11 protein; Molybdenum cofactor synthesis-step 1 protein A-B; Molybdenum cofactor biosynthesis protein A; Molybdenum cofactor biosynthesis protein C) (Azide free) (; Molybdenum cofactor biosynthesis protein C; anti-MOCS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 385

• Applicable Applications for anti-MOCS1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-482 amino acids from the C-terminal region of human MOCS1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MOCS1 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MOCS1 antibody detected the MOCS1 protein (arrow).)

Immunohistochemistry (IHC)

(MOCS1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MOCS1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MOCS1 antibody

MONDOA forms heterodimers with MLX (MIM 602976) that can bind to and activate transcription from CACGTG E boxes (Billin et al., 2000 [PubMed 11073985]).

Product Categories/Family for anti-MOCS1 antibody

Antibodies; Transcription Factors

References

Peterson, C.W., et al. Mol. Cell. Biol. 30(12):2887-2895(2010). Kaadige, M.R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(35):14878-14883(2009). Stoltzman, C.A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(19):6912-6917(2008). Sans, C.L., et al. Mol. Cell. Biol. 26(13):4863-4871(2006). Bornhauser, B.C., et al. J. Biol. Chem. 278(37):35412-35420(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 22027536
• NCBI GeneID: 4337
• NCBI Accession #: NP_005934.2
• NCBI GenBank Nucleotide #: NP_005934.2
• UniProt Accession #: Q9NZB8
• NCBI Official Full Name: molybdenum cofactor biosynthesis protein 1 isoform 1
• NCBI Official Synonym Full Names: molybdenum cofactor synthesis 1
• NCBI Official Symbol: MOCS1
• NCBI Official Synonym Symbols: MIG11; MOCOD; MOCS1A; MOCS1B
• NCBI Protein Information: molybdenum cofactor biosynthesis protein 1
• UniProt Protein Name: Molybdenum cofactor biosynthesis protein 1
• UniProt Gene Name: MOCS1
• UniProt Entry Name: MOCS1_HUMAN

NCBI Description

Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However, only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA, whereas additional splice variants are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency, type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq, Nov 2017]

Uniprot Description

Function: Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z). Ref.8

Catalytic activity: GTP = cyclic pyranopterin phosphate + diphosphate. Ref.8

Cofactor: Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate. Ref.10

Pathway: Cofactor biosynthesis; molybdopterin biosynthesis.

Subunit structure: Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer

Probable.

Tissue specificity: Isoform MOCS1A and isoform 2 are widely expressed. Ref.1 Ref.9

Post-translational modification: Isoform MOCS1A, isoform 2 and isoform 3 are probably thiocarboxylated at their C-terminus. Thiocarboxylation probably plays a central role in molybdenum cofactor biosynthesis, since mutagenesis of the last 2 Gly residues of isoform MOCS1A abolishes the catalytic activity of the enzyme. Thiocarboxylation is absent in isoform MOCS1B, which lacks the C-terminal Gly residue.

Involvement in disease: Molybdenum cofactor deficiency type A (MOCOD type A) [MIM:252150]: Autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: The MOCS1 locus has initially been reported to produce MOCS1A and MOCS1B from non-overlapping reading frames within a bicistronic transcript. However, only isoform MOCS1A seems to be translated from the bicistronic transcript. Isoform MOCS1B seems to be translated from a monocistronic mRNA that is derived by alternative splicing.

Sequence similarities: In the C-terminal section; belongs to the MoaC family.In the N-terminal section; belongs to the MoaA/NifB/PqqE family.

Sequence caution: The sequence AAB87524.1 differs from that shown. Reason: Alternative splicing in the MOCS1A-MOCS1B joining region.The sequence AAS00489.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC44526.1 differs from that shown. Reason: Alternative splicing in the MOCS1A-MOCS1B joining region.The sequence CAI20007.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI20012.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI20013.1 differs from that shown. Reason: Erroneous gene model prediction.