Wnt-9b active protein

Human Wnt-9b

Cat. Num. AAA692303

• Gene Names: WNT9B; WNT15; WNT14B
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & HPLC analysis
• Synonyms: Wnt-9b; Human Wnt-9b; WNT15; WNT14B; UNQ6973/PRO21956; Wnt-9b active protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & HPLC analysis
• Form/Format: Lyophilized
• Sequence: SYFGLTGREV LTPFPGLGTA AAPAQGGAHL KQCDLLKLSR RQKQLC RREP GLAETLRDAA HLGLLECQFQ FRHERWNCSL EGRMGLLKRG F KETAFLYAV SSAALTHTLA RACSAGRMER CTCDDSPGLE SRQAWQW GVC GDNLKYSTKF LSNFLGSKRG NKDLRARADA HNTHVGIKAV KS GLRTTCKC HGVSGSCAVR TCWKQLSPFR ETGQVLKLRY DSAVKVSS AT NEALGRLELW APARQGSLTK GLAPRSGDLV YMEDSPSFCR PSK YSPGTAG RVCSREASCS SLCCGRGYDT QSRLVAFSCH CQVQWCCYV E CQQCVQEELV YTCKH
• Sequence Length: 357

• Biological Activity: Determined by its ability to induce alkaline phosphatase production by CCL-226 cells.

Related Product Information for Wnt-9b active protein

Formerly known as Wnt-15 or Wnt-14b, Wnt-9b is a secreted glycoprotein belonging to the Wnt family of signaling proteins that are critically involved in maintaining the integrity of both embryonic and adult tissues. Wnt-9b is primarily expressed in adult kidneys and during late embryogenesis, and shares with other Wnt family members the same highly conserved lipid-modified, cysteine-rich domain essential for cell signaling. As is true for most Wnt family members, Wnt-9b functions through the biochemical process known as the canonical Wnt pathway; during which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family, and ultimately result in the disruption of beta-catenin degradation. Intracellular accumulation of beta-catenin increases translocation of the protein into the nucleus, where it binds to TCF/LEF transcription factors to promote the expression of numerous genes. In this manner, Wnt signaling induces and maintains transformed phenotype, and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. While increased Wnt/beta-catenin signaling is associated with tumorigenesis in a diverse set of human cancers, lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes, and has been shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Altered Wnt-9b expression has been shown to result in the underdevelopment of the kidneys, and incomplete lip and cleft fusion in mice. Recombinant Human Wnt-9b is a monomeric glycoprotein containing 335 amino acid residues and has a calculated molecular weight of 36.9 kDa. Due to glycosylation, Recombinant Human Wnt-9b migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.

Product Categories/Family for Wnt-9b active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 17017976
• NCBI GeneID: 7484
• NCBI Accession #: NP_003387.1
• NCBI GenBank Nucleotide #: NM_003396.2
• UniProt Accession #: O14905; Q6UXT4; Q96Q09
• Molecular Weight: 39,001 Da
• NCBI Official Full Name: protein Wnt-9b isoform 1
• NCBI Official Synonym Full Names: Wnt family member 9B
• NCBI Official Symbol: WNT9B
• NCBI Official Synonym Symbols: WNT15; WNT14B
• NCBI Protein Information: protein Wnt-9b
• UniProt Protein Name: Protein Wnt-9b
• UniProt Gene Name: WNT9B
• UniProt Synonym Gene Names: WNT14B; WNT15

NCBI Description

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

WNT9B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: extracellular region; extracellular space

Molecular Function: frizzled binding

Biological Process: cell fate commitment; neuron differentiation; Wnt receptor signaling pathway