Typical Testing Data/Standard Curve (for reference only)
Wingless-type MMTV integration site family, member 9B ELISA Kit | WNT9B elisa kit
Rat Wingless-type MMTV integration site family, member 9B ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: WNT9B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-WNT9B antibody and an WNT9B-HRP conjugate. The assay sample and buffer are incubated together with WNT9B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WNT9B concentration since WNT9B from samples and WNT9B-HRP conjugate compete for the anti-WNT9B antibody binding site. Since the number of sites is limited, as more sites are occupied by WNT9B from the sample, fewer sites are left to bind WNT9B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WNT9B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT9B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region
Molecular Function: frizzled binding
Biological Process: positive regulation of catalytic activity; regulation of protein amino acid phosphorylation; cell fate commitment; response to retinoic acid; Wnt receptor signaling pathway; regulation of asymmetric cell division; Wnt receptor signaling pathway, planar cell polarity pathway; in utero embryonic development; multicellular organismal development; embryonic cranial skeleton morphogenesis; Wnt receptor signaling pathway through beta-catenin; palate development; cellular response to starvation; neuron differentiation; cell-cell signaling; ureteric bud branching; male genitalia development; regulation of tube size
Research Articles on WNT9B
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Product Notes
The Rat WNT9B wnt9b (Catalog #AAA744672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744672 ELISA Kit recognizes Rat WNT9B. It is sometimes possible for the material contained within the vial of "Wingless-type MMTV integration site family, member 9B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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