CD100, soluble Active Protein | CD100 active protein

Human CD100, soluble

Cat. Num. AAA691768

• Gene Names: SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• Reactivity: Human
• Purity: > 97%, by SDS-PAGE and HPLC analysis
• Synonyms: CD100; soluble; Human CD100; Recombinant Human soluble CD100; CD100 active protein

• Host: CHO Cells
• Reactivity: Human
• Purity/Purification: > 97%, by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREA VFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR V LQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDP AHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PW LNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIP RI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDV FVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKY M QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLP DKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLL SSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHG GTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN L SEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSV VQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TG TSCEPKIV INTVPQLHSE KTMYLKSSDN R
• Sequence Length: 738

• Endotoxin Level: < 0.1 ng per ug of sCD100
• Biological Activity: Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.

Related Product Information for CD100 active protein

The Semaphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC "cooperation". The CD100 precursor contains 862 amino acids, including a 21 a.a. signal sequence, a 713 a.a. extracellular domain, a 21 a.a. transmembrane sequence, and a 107 a.a cytoplasmic region. The extracellular sequence contains several structural features, including a 479 a.a "sema" domain, a 79 a.a. Ig-like sequence, and a 52 a.a "Plexin-type repeat". Recombinant soluble CD100 (sCD100) is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.

Product Categories/Family for CD100 active protein

Soluble receptors

NCBI and Uniprot Product Information

• NCBI GI #: 214010220
• NCBI GeneID: 10507
• NCBI Accession #: NP_001135759.1
• NCBI GenBank Nucleotide #: NM_001142287.1
• UniProt Accession #: Q92854; Q7Z5S4; Q8N8B0; B2RPM6
• Molecular Weight: 78.9 kDa
• NCBI Official Full Name: semaphorin-4D isoform 2
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
• UniProt Protein Name: Semaphorin-4D
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ
• UniProt Entry Name: SEM4D_HUMAN

Uniprot Description

Function: Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA. Ref.1 Ref.6 Ref.8 Ref.11

Subunit structure: Homodimer. Interacts with PLXNB2

By similarity. Binds PLXNB1. Ref.5 Ref.11

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.1 Ref.11.

Tissue specificity: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. Ref.1

Sequence similarities: Belongs to the semaphorin family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 PSI domain.Contains 1 Sema domain.

Sequence caution: The sequence AAI37516.1 differs from that shown. Reason: Cloning artifact.The sequence AAI37519.1 differs from that shown. Reason: Cloning artifact.The sequence BAC04938.1 differs from that shown. Reason: Cloning artifact.The sequence CAI95794.1 differs from that shown. Reason: Erroneous gene model prediction.