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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD100:APC (MBS214898))

Mouse CD100 Monoclonal Antibody | anti-CD100 antibody

MOUSE ANTI HUMAN CD100:FITC

Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Applications
Flow Cytometry, Functional Assay
Synonyms
CD100; Monoclonal Antibody; MOUSE ANTI HUMAN CD100:FITC; anti-CD100 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A8
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid from tissue culture supernatant.
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
738
Applicable Applications for anti-CD100 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Immunogen
Human PHA activated lymphocytes
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD100:APC (MBS214898))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD100:APC (MBS214898))
Related Product Information for anti-CD100 antibody
Mouse anti Human CD100 antibody, clone A8 recognizes the human CD100 cell surface antigen, a 150kDa homodimeric glycoprotein expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,164 Da
NCBI Official Full Name
semaphorin-4D isoform 2
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; BB18; GR3; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: axon guidance; negative regulation of peptidyl-tyrosine phosphorylation; regulation of dendrite morphogenesis; negative regulation of cell adhesion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of osteoblast differentiation; immune response; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; regulation of cell projection organization and biogenesis; positive regulation of cell migration; negative regulation of apoptosis

Research Articles on CD100

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Product Notes

The CD100 sema4d (Catalog #AAA210643) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD100 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD100 sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD100, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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