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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.)

Mouse CD103 Monoclonal Antibody | anti-CD103 antibody

Anti-Hu CD103 FITC

Gene Names
ITGAE; CD103; HUMINAE
Reactivity
Human, Non-human primates
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD103; Monoclonal Antibody; Anti-Hu CD103 FITC; integrin subunit alpha E; Integrin alpha E; ITGAE; anti-CD103 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Non-human primates
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
Ber-ACT8
Specificity
The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.
Purity/Purification
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
1179
Applicable Applications for anti-CD103 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
HTLV-1 induced human T cell line MAPS16
Conjugation
FITC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.)
Related Product Information for anti-CD103 antibody
CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Integrin alpha-E
NCBI Official Synonym Full Names
integrin subunit alpha E
NCBI Official Symbol
ITGAE
NCBI Official Synonym Symbols
CD103; HUMINAE
NCBI Protein Information
integrin alpha-E
UniProt Protein Name
Integrin alpha-E
UniProt Gene Name
ITGAE
UniProt Entry Name
ITAE_HUMAN

NCBI Description

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAE: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: plasma membrane; integrin complex; external side of plasma membrane

Molecular Function: metal ion binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; cell adhesion

Research Articles on CD103

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Product Notes

The CD103 itgae (Catalog #AAA1800419) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD103 FITC reacts with Human, Non-human primates and may cross-react with other species as described in the data sheet. AAA Biotech's CD103 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD103 itgae for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD103, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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