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TLR3 active protein

TLR3

Applications
SDS-Page
Purity
>95%, as determined by SDS-PAGE and HPLC.
Synonyms
TLR3; TLR3 active protein
Ordering
For Research Use Only!
Host
CHO
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Form/Format
Lyophilized; PBS, pH 7.2
Sequence
STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLE LSSNQIKEFS PGCFHAIGRL FGLFLNNVQL GPSLTEKLCL ELANTSIRNL SLSNSQLSTT SNTTFLGLKW TNLTMLDLSY NNLNVVGNDS FAWLPQLEYF FLEYNNIQHL FSHSLHGLFN VRYLNLKRSF TKQSISLASL PKIDDFSFQW LKCLEHLNME DNDIPGIKSN MFTGLINLKY LSLSNSFTSL RTLTNETFVS LAHSPLHILN LTKNKISKIE SDAFSWLGHL EVLDLGLNEI GQELTGQEWR GLENIFEIYL SYNKYLQLTR NSFALVPSLQ RLMLRRVALK NVDSSPSPFQ PLRNLTILDL SNNNIANIND DMLEGLEKLE ILDLQHNNLA RLWKHANPGG PIYFLKGLSH LHILNLESNG FDEIPVEVFK DLFELKIIDL GLNNLNTLPA SVFNNQVSLK SLNLQKNLIT SVEKKVFGPA FRNLTELDMR FNPFDCTCES IAWFVNWINE THTNIPELSS HYLCNTPPHY HGFPVRLFDT SSCKDSAPFE L
Sequence Length
92
Applicable Applications for TLR3 active protein
SDS-PAGE
Application Notes
Recombinant TLR3 migrates as an approximately 75 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Species
Human
Source Note
Optimized DNA sequence encoding Human TLR3 mature chain was expressed in CHO. Recombinant Human Toll-like receptor 3 encodes amino acid residues 24-904.
Biological Activity
Determined by its ability to neutralize Poly I:C induced NF-B signaling in HT-29 cells stably transfected with NF-B-luc. The ED50 for this effect is 5-10 ug/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
10,341 Da
NCBI Official Full Name
TLR3, partial
UniProt Protein Name
TLR3
Protein Family
UniProt Gene Name
TLR3

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Product Notes

The TLR3 tlr3 (Catalog #AAA553629) is an Active Protein produced from CHO and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TLR3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Recombinant TLR3 migrates as an approximately 75 kDa protein due to glycosylation under reducing conditions in SDS-PAGE. Researchers should empirically determine the suitability of the TLR3 tlr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLE LSSNQIKEFS PGCFHAIGRL FGLFLNNVQL GPSLTEKLCL ELANTSIRNL SLSNSQLSTT SNTTFLGLKW TNLTMLDLSY NNLNVVGNDS FAWLPQLEYF FLEYNNIQHL FSHSLHGLFN VRYLNLKRSF TKQSISLASL PKIDDFSFQW LKCLEHLNME DNDIPGIKSN MFTGLINLKY LSLSNSFTSL RTLTNETFVS LAHSPLHILN LTKNKISKIE SDAFSWLGHL EVLDLGLNEI GQELTGQEWR GLENIFEIYL SYNKYLQLTR NSFALVPSLQ RLMLRRVALK NVDSSPSPFQ PLRNLTILDL SNNNIANIND DMLEGLEKLE ILDLQHNNLA RLWKHANPGG PIYFLKGLSH LHILNLESNG FDEIPVEVFK DLFELKIIDL GLNNLNTLPA SVFNNQVSLK SLNLQKNLIT SVEKKVFGPA FRNLTELDMR FNPFDCTCES IAWFVNWINE THTNIPELSS HYLCNTPPHY HGFPVRLFDT SSCKDSAPFE L . It is sometimes possible for the material contained within the vial of "TLR3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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