Western Blot (WB)
(Western blot analysis of Myosin 14 expression in Hela (A), A431 (B) whole cell lysates.)
Rabbit anti-Human, Mouse Myosin 14 Polyclonal Antibody | anti-MYH14 antibody
Anti-Myosin 14 Antibody
Immunohistochemistry: (1/100 - 1/200)
Upon delivery aliquot and store at -20°C for one year.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Myosin 14 expression in Hela (A), A431 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Myosin 14 expression in Hela (A), A431 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Myosin 14 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Myosin 14 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
MYH14: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). High levels of expression are found in small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: actomyosin; axon; brush border; cytosol; growth cone; membrane; myelin sheath; myosin II complex; stress fiber
Molecular Function: actin filament binding; actin-dependent ATPase activity; ATP binding; calmodulin binding; microfilament motor activity
Biological Process: actin filament-based movement; actomyosin structure organization and biogenesis; axon guidance; ephrin receptor signaling pathway; generation of action potential; metabolic process; regulation of cell shape; sensory perception of sound; skeletal muscle contraction; skeletal muscle development; small GTPase mediated signal transduction
Disease: Deafness, Autosomal Dominant 4a; Peripheral Neuropathy, Myopathy, Hoarseness, And Hearing Loss
Research Articles on MYH14
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Product Notes
The MYH14 myh14 (Catalog #AAA8242946) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Myosin 14 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin 14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000) Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the MYH14 myh14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin 14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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