Magnesium-chelatase subunit chlI, chloroplastic (CHLI) Recombinant Protein | CHLI recombinant protein

Recombinant Arabidopsis thaliana Magnesium-chelatase subunit chlI, chloroplastic (CHLI)

Cat. Num. AAA1097804

• Gene Names: CHLI1; CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Magnesium-chelatase subunit chlI; chloroplastic (CHLI); Recombinant Arabidopsis thaliana Magnesium-chelatase subunit chlI; CHLI recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 61-424, Full length protein
• Sequence: VMNVATEINSTEQVVGKFDSKKSARPVYPFAAIVGQDEMKLCLLLNVIDPKIGGVMIMGDRGTGKSTTVRSLVDLLPEINVVAGDPYNSDPIDPEFMGVEVRERVEKGEQVPVIATKINMVDLPLGATEDRVCGTIDIEKALTEGVKAFEPGLLAKANRGILYVDEVNLLDDHLVDVLLDSAASGWNTVEREGISISHPARFILIGSGNPEEGELRPQLLDRFGMHAQVGTVRDADLRVKIVEERARFDSNPKDFRDTYKTEQDKLQDQISTARANLSSVQIDRELKVKISRVCSELNVDGLRGDIVTNRAAKALAALKGKDRVTPDDVATVIPNCLRHRLRKDPLESIDSGVLVSEKFAEIFS
• Sequence Length: 364

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15233888
• NCBI GeneID: 827580
• NCBI Accession #: NP_193583.1
• NCBI GenBank Nucleotide #: NM_117962.3
• UniProt Accession #: P16127; Q0WUK7
• Molecular Weight: 46,270 Da
• NCBI Official Full Name: P-loop containing nucleoside triphosphate hydrolases superfamily protein
• NCBI Official Symbol: CHLI1
• NCBI Official Synonym Symbols: CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
• NCBI Protein Information: P-loop containing nucleoside triphosphate hydrolases superfamily protein
• UniProt Protein Name: Magnesium-chelatase subunit ChlI-1, chloroplastic
• Protein Family: Magnesium-chelatase
• UniProt Gene Name: CHLI1
• UniProt Synonym Gene Names: CH42; CS; Mg-chelatase subunit I-1

NCBI Description

Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.

Uniprot Description

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and CHLH. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. Possesses high affinity for ATP and may play a major role in chlorophyll biosynthesis. Does not bind abscisic acid (ABA), but is a positive regulator of ABA signaling.