Rabbit CHLI1 Polyclonal Antibody | anti-CHLI1 antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHLI1 Polyclonal Antibody

• Gene Names: CHLI1; CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: CHLI1; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHLI1 Polyclonal Antibody; CHLI1 Antibody; Magnesium-chelatase subunit ChlI-1; chloroplastic; Mg-chelatase subunit I-1; EC 6.6.1.1; Mg-protoporphyrin IX chelatase subunit ChlI-1; Protein CHLORINA 42; CHLI1 CH42 CS At4g18480 F28J12.140; anti-CHLI1 antibody

• Host: Rabbit
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-CHLI1 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Arabidopsis thaliana CHLI1 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 15233888
• NCBI GeneID: 827580
• NCBI Accession #: NP_193583.1
• NCBI GenBank Nucleotide #: NM_117962.3
• UniProt Accession #: P16127; Q0WUK7
• Molecular Weight: 46,270 Da
• NCBI Official Full Name: P-loop containing nucleoside triphosphate hydrolases superfamily protein
• NCBI Official Symbol: CHLI1
• NCBI Official Synonym Symbols: CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
• NCBI Protein Information: P-loop containing nucleoside triphosphate hydrolases superfamily protein
• UniProt Protein Name: Magnesium-chelatase subunit ChlI-1, chloroplastic
• Protein Family: Magnesium-chelatase
• UniProt Gene Name: CHLI1
• UniProt Synonym Gene Names: CH42; CS; Mg-chelatase subunit I-1

NCBI Description

Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.

Uniprot Description

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and CHLH. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. Possesses high affinity for ATP and may play a major role in chlorophyll biosynthesis. Does not bind abscisic acid (ABA), but is a positive regulator of ABA signaling.