Epithelial Cell Transforming Sequence 2 (ECT2) Recombinant Protein | ECT2 recombinant protein

Recombinant Epithelial Cell Transforming Sequence 2 (ECT2)

Cat. Num. AAA2010503

• Gene Names: ECT2; ARHGEF31
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Epithelial Cell Transforming Sequence 2 (ECT2); Recombinant Epithelial Cell Transforming Sequence 2 (ECT2); ECT2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LVHHM GGVIRKDFNS KVTHLVANCT QGEKFRVAVS LGTPIMKPEW IYKAWERRNE QDFYAAVDDF RNEFKVPPFQ DCILSFLGFS DEEKTNMEEM TEMQGGKYLP LGDERCTHLV VEENIVKDLP FEPSKKLYVV KQEWFWGSIQ MDARAGETMY LYEKANTPEL KKSVSMLSLN TPNSNRKR
• Sequence Length: 914

• Applicable Applications for ECT2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu166~Arg348 (Accession # Q9H8V3) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 385198079
• NCBI GeneID: 1894
• NCBI Accession #: NP_001245244.1
• NCBI GenBank Nucleotide #: NM_001258315.1
• UniProt Accession #: Q9H8V3; Q0MT80; Q2M269; Q6U836; Q9NSV8; Q9NVW9
• Molecular Weight: 22.8kDa
• NCBI Official Full Name: protein ECT2 isoform a
• NCBI Official Synonym Full Names: epithelial cell transforming sequence 2 oncogene
• NCBI Official Symbol: ECT2
• NCBI Official Synonym Symbols: ARHGEF31
• NCBI Protein Information: protein ECT2; epithelial cell-transforming sequence 2 oncogene
• UniProt Protein Name: Protein ECT2
• Protein Family: Protein
• UniProt Gene Name: ECT2
• UniProt Entry Name: ECT2_HUMAN

NCBI Description

The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

ECT2: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)- induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q26.1-q26.2

Cellular Component: nuclear membrane; tight junction; intracellular membrane-bound organelle; cytoplasm; midbody; intercellular junction; nucleus; cytosol; cleavage furrow

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; signal transducer activity; Rho GTPase binding; protein homodimerization activity; GTPase activator activity

Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; cell morphogenesis; cytokinesis; regulation of small GTPase mediated signal transduction; protein transport; positive regulation of protein import into nucleus; small GTPase mediated signal transduction; activation of protein kinase activity; regulation of protein kinase activity; positive regulation of neuron differentiation; protein homooligomerization