E3 ubiquitin-protein ligase CHIP (CHIP) Recombinant Protein | CHIP recombinant protein

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase CHIP (CHIP)

Cat. Num. AAA1407909

• Gene Names: CHIP; ATCHIP; carboxyl terminus of HSC70-interacting protein; CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase CHIP (CHIP); Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase CHIP (CHIP); CHIP recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-278, Full length protein
• Sequence: MVTGVASAMAERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRKDWTKVEEDCRKAIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYMVEEIWEELSKAKYMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALERVFKKAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKVGKFDPITREKIDPANLVPNLAIKEAVAAYLEKHVWAYKMGC
• Sequence Length: 278

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 18397925
• NCBI GeneID: 819925
• NCBI Accession #: NP_566305.1
• NCBI GenBank Nucleotide #: NM_111616.3
• UniProt Accession #: Q9SRS9
• Molecular Weight: 31,655 Da
• NCBI Official Full Name: carboxyl terminus of HSC70-interacting protein
• NCBI Official Symbol: CHIP
• NCBI Official Synonym Symbols: ATCHIP; carboxyl terminus of HSC70-interacting protein; CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN
• NCBI Protein Information: carboxyl terminus of HSC70-interacting protein
• UniProt Protein Name: E3 ubiquitin-protein ligase CHIP
• Protein Family: Chitoporin
• UniProt Gene Name: CHIP
• UniProt Synonym Gene Names: AtCHIP

NCBI Description

Encodes AtCHIP, a new class of E3 ubiquitin ligases with three tetratricopeptide repeats and a U-box domain, structurally similar to the animal CHIP proteins. Plays an important role in plant cellular metabolism under temperature stress conditions. Functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment. Belongs to one of the 36 carboxylate clamp (CC)-tetratricopeptide repeat (TPR) proteins (Prasad 2010, Pubmed ID: 20856808) with potential to interact with Hsp90/Hsp70 as co-chaperones.

Uniprot Description

Has E3 ubiquitin-protein ligase activity and may target misfolded substrates towards proteasomal degradation. Regulates the activity of some serine/threonine-protein phosphatases by E3 ubiquitin-protein ligase activity. Required for responses to biotic and abiotic stresses such as auxin, abscisic acid (ABA), low and high temperature and darkness, probably through the activation of serine/threonine-protein phosphatase and the subsequent modification of the plasma membrane composition. Regulates the chloroplastic Clp proteolytic activity in response to stresses. Ubiquitylates FtsH1, a component of the chloroplast FtsH protease, and affects protein degradation in chloroplasts. Mediates plastid precursor degradation to prevent cytosolic precursor accumulation, together with the molecular chaperone HSC70-4. Mediates ubiquitination of transit peptides and thereby led to their degradation through the ubiquitin-proteasome system.