Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) Standard Curve.)
Human Ras-Specific Guanine Nucleotide-Releasing Factor RalGPS1 (RALGPS1) ELISA Kit | RALGPS1 elisa kit
Human Ras-Specific Guanine Nucleotide-Releasing Factor RalGPS1 (RALGPS1) ELISA Kit
Gene Names
RALGPS1; RALGEF2; RALGPS1A
Reactivity
Human
Synonyms
Ras-Specific Guanine Nucleotide-Releasing Factor RalGPS1 (RALGPS1); Human Ras-Specific Guanine Nucleotide-Releasing Factor RalGPS1 (RALGPS1) ELISA Kit; RALGPS1; RP13-225O21.1; KIAA0351; RALGEF2; RALGPS1A; Ral guanine nucleotide exchange factor RalGPS1A; RALGPS1 elisa kit
Reactivity
Human
Specificity
Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RALGPS1. No significant cross-reactivity or interference between Human RALGPS1 and analogues was observed.
Sequence Length
305
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) Standard Curve.)
Related Product Information for RALGPS1 elisa kit
Description: This Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit employs a two-site sandwich ELISA to quantitate RALGPS1 in samples. An antibody specific for RALGPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RALGPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RALGPS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RALGPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RalGPS1 is a Ral GEF that requires membrane targeting to function in vivo.RalGPS 1 is a highly specific Ral GEF. Guanine nucleotide exchange factors (GEFs) are responsible for coupling cell surface receptors to Ras protein activation. These molecules share the core catalytic domain of other Ras family GEFs but lack the catalytic non-conserved domain that is believed to contribute to Sos1 integrity. In vitro binding and in vivo nucleotide exchange assays indicate that these GEFs specifically catalyze the GTP loading of the Ral GTPase when overexpressed in 293T cells. A central proline-rich motif associated with the Src homology (SH)2/SH3-containing adapter proteins Grb2 and Nck in vivo, whereas a pleckstrin homology (PH) domain was located at the GEF C terminus.
Background: RalGPS1 is a Ral GEF that requires membrane targeting to function in vivo.RalGPS 1 is a highly specific Ral GEF. Guanine nucleotide exchange factors (GEFs) are responsible for coupling cell surface receptors to Ras protein activation. These molecules share the core catalytic domain of other Ras family GEFs but lack the catalytic non-conserved domain that is believed to contribute to Sos1 integrity. In vitro binding and in vivo nucleotide exchange assays indicate that these GEFs specifically catalyze the GTP loading of the Ral GTPase when overexpressed in 293T cells. A central proline-rich motif associated with the Src homology (SH)2/SH3-containing adapter proteins Grb2 and Nck in vivo, whereas a pleckstrin homology (PH) domain was located at the GEF C terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ras-specific guanine nucleotide-releasing factor RalGPS1 isoform 4
NCBI Official Synonym Full Names
Ral GEF with PH domain and SH3 binding motif 1
NCBI Official Symbol
RALGPS1
NCBI Official Synonym Symbols
RALGEF2; RALGPS1A
NCBI Protein Information
ras-specific guanine nucleotide-releasing factor RalGPS1
UniProt Protein Name
Ras-specific guanine nucleotide-releasing factor RalGPS1
Protein Family
UniProt Gene Name
RALGPS1
UniProt Synonym Gene Names
KIAA0351; RALGEF2; RalGEF 2
UniProt Entry Name
RGPS1_HUMAN
Research Articles on RALGPS1
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Product Notes
The Human RALGPS1 ralgps1 (Catalog #AAA9715998) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715998 ELISA Kit recognizes Human RALGPS1. It is sometimes possible for the material contained within the vial of "Ras-Specific Guanine Nucleotide-Releasing Factor RalGPS1 (RALGPS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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