Rabbit anti-Human SGOL1 Polyclonal Antibody | anti-SGOL1 antibody

SGOL1, ID (SGOL1, SGO1, Shugoshin-like 1, Serologically defined breast cancer antigen NY-BR-85) (PE)

Cat. Num. AAA6347042

• Gene Names: SGOL1; SGO; Sgo1; NY-BR-85
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SGOL1; Polyclonal Antibody; ID (SGOL1; SGO1; Shugoshin-like 1; Serologically defined breast cancer antigen NY-BR-85) (PE); Serologically defined breast cancer antigen NY-BR-85; anti-SGOL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SGOL1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SGOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-367 amino acids from the Central region of human SGOL1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SGOL1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SGOL1 antibody detected the SGOL1 protein (arrow).)

Related Product Information for anti-SGOL1 antibody

SGOL1 plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.

Product Categories/Family for anti-SGOL1 antibody

Antibodies; Cancer Markers

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Okamoto, N., et al. Genes Cells 15(5):471-484(2010). Xu, Z., et al. Mol. Cell 35(4):426-441(2009). Gambe, A.E., et al. FEBS Lett. 583(12):1951-1956(2009). Karamysheva, Z., et al. J. Biol. Chem. 284(3):1772-1780(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 60302875
• NCBI GeneID: 151648
• NCBI Accession #: NP_001012409.1; NP_001012410.1
• NCBI GenBank Nucleotide #: NM_001012409.2
• UniProt Accession #: Q5FBB7; Q588H5; Q5FBB4; Q5FBB5; Q5FBB6; Q5FBB8; Q8N579; Q8WVL0; Q9BVA8; Q9H275
• Molecular Weight: 64,190 Da
• NCBI Official Full Name: shugoshin-like 1 isoform A1
• NCBI Official Synonym Full Names: shugoshin-like 1 (S. pombe)
• NCBI Official Symbol: SGOL1
• NCBI Official Synonym Symbols: SGO; Sgo1; NY-BR-85
• NCBI Protein Information: shugoshin-like 1; hSgo1; shugoshin 1AB protein; shugoshin 1CD protein; shugoshin 1EF protein; shugoshin 1GH protein; shugoshin 1KL protein; serologically defined breast cancer antigen NY-BR-85
• UniProt Protein Name: Shugoshin-like 1
• UniProt Gene Name: SGOL1
• UniProt Synonym Gene Names: SGO1; hSgo1
• UniProt Entry Name: SGOL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

SGO1: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Belongs to the shugoshin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: kinetochore; nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region; chromosome, pericentric region

Molecular Function: protein binding; kinase binding

Biological Process: mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; centriole-centriole cohesion; meiotic chromosome segregation; chromosome segregation

Disease: Chronic Atrial And Intestinal Dysrhythmia