Human Vascular endothelial growth factor C ELISA Kit | VEGFC elisa kit

Human Vascular endothelial growth factor C ELISA Kit

Cat. Num. AAA2886346

• Gene Names: VEGFC; VRP; Flt4-L; LMPH1D
• Reactivity: Human
• Synonyms: Vascular endothelial growth factor C; Human Vascular endothelial growth factor C ELISA Kit; VEGF-C; Flt4 ligand; Flt4-L; Vascular endothelial growth factor-related protein; VRP; VEGFC; VEGFC elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Vascular endothelial growth factor C,VEGF-C
• Sequence Length: 419

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.056 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=6.3%
• Inter-assay Precision: Inter-Assay CV: <=10.6%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFC elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.4
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L; LMPH1D
• NCBI Protein Information: vascular endothelial growth factor C
• UniProt Protein Name: Vascular endothelial growth factor C
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: extracellular region; extracellular space; membrane

Molecular Function: chemoattractant activity; growth factor activity; protein binding; vascular endothelial growth factor receptor 3 binding

Biological Process: angiogenesis; blood coagulation; induction of positive chemotaxis; morphogenesis of embryonic epithelium; negative regulation of blood pressure; negative regulation of cell proliferation; organ morphogenesis; platelet activation; platelet degranulation; positive chemotaxis; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of epithelial cell proliferation; positive regulation of neuroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid autophosphorylation; positive regulation of protein secretion; regulation of vascular endothelial growth factor receptor signaling pathway; response to drug; signal transduction; substrate-bound cell migration; vascular endothelial growth factor receptor signaling pathway

Disease: Lymphedema, Hereditary, Id