Chicken thrombospondin 1 (TSP-1) ELISA Kit | TSP-1 elisa kit

Chicken thrombospondin 1 (TSP-1) ELISA Kit

Cat. Num. AAA263628

• Gene Names: THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
• Reactivity: Chicken
• Synonyms: thrombospondin 1 (TSP-1); Chicken thrombospondin 1 (TSP-1) ELISA Kit; TSP-1 elisa kit

• Reactivity: Chicken
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1170

• Samples: Chicken serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TSP-1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken TSP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 40317626
• NCBI GeneID: 7057
• NCBI Accession #: NP_003237.2
• NCBI GenBank Nucleotide #: NM_003246.2
• Molecular Weight: 120,148 Da
• NCBI Official Full Name: thrombospondin-1
• NCBI Official Synonym Full Names: thrombospondin 1
• NCBI Official Symbol: THBS1
• NCBI Official Synonym Symbols: TSP; THBS; TSP1; TSP-1; THBS-1
• NCBI Protein Information: thrombospondin-1; thrombospondin-1p180
• UniProt Protein Name: Thrombospondin-1
• Protein Family: Tetraspanin
• UniProt Gene Name: THBS1
• UniProt Synonym Gene Names: TSP; TSP1
• UniProt Entry Name: TSP1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.

Protein type: Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; fibrinogen complex; extracellular region; external side of plasma membrane; secretory granule

Molecular Function: heparin binding; identical protein binding; laminin binding; calcium ion binding; integrin binding; protein binding; proteoglycan binding; fibroblast growth factor binding; phosphatidylserine binding; transforming growth factor beta binding; fibronectin binding; low-density lipoprotein binding; glycoprotein binding

Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell adhesion; cell cycle arrest; positive regulation of macrophage activation; response to drug; platelet activation; negative regulation of interleukin-12 production; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; response to testosterone stimulus; negative regulation of blood vessel endothelial cell migration; negative regulation of cell-matrix adhesion; response to unfolded protein; positive regulation of angiogenesis; response to mechanical stimulus; negative regulation of endothelial cell proliferation; regulation of cGMP metabolic process; peptide cross-linking; response to progesterone stimulus; response to calcium ion; negative regulation of apoptosis; positive regulation of blood coagulation; positive regulation of translation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; platelet degranulation; positive regulation of transforming growth factor-beta1 production; protein amino acid O-linked glycosylation; positive regulation of tumor necrosis factor biosynthetic process; cell migration; chronic inflammatory response; negative regulation of focal adhesion formation; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; post-translational protein modification; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; cellular protein metabolic process; negative regulation of dendritic cell antigen processing and presentation; response to hypoxia; immune response; sprouting angiogenesis; blood coagulation; positive regulation of phosphorylation; positive regulation of cell migration