Typical Testing Data/Standard Curve (for reference only)
Rat TSP-1 ELISA Kit | TSP-1 elisa kit
Rat TSP-1(Thrombospondin 1)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TSP-1. You can calculate the concentration of TSP-1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.
Protein type: Motility/polarity/chemotaxis; Inhibitor
Chromosomal Location of Human Ortholog: 15q15
Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; fibrinogen complex; extracellular region; secretory granule; external side of plasma membrane
Molecular Function: heparin binding; identical protein binding; laminin binding; calcium ion binding; integrin binding; protein binding; proteoglycan binding; fibroblast growth factor binding; transforming growth factor beta binding; phosphatidylserine binding; fibronectin binding; low-density lipoprotein binding; glycoprotein binding
Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell adhesion; cell cycle arrest; positive regulation of macrophage activation; response to drug; platelet activation; negative regulation of interleukin-12 production; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; response to testosterone stimulus; negative regulation of cell-matrix adhesion; negative regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of angiogenesis; response to mechanical stimulus; negative regulation of endothelial cell proliferation; regulation of cGMP metabolic process; peptide cross-linking; response to progesterone stimulus; response to calcium ion; negative regulation of apoptosis; positive regulation of blood coagulation; positive regulation of translation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; platelet degranulation; protein amino acid O-linked glycosylation; positive regulation of transforming growth factor-beta1 production; positive regulation of tumor necrosis factor biosynthetic process; cell migration; chronic inflammatory response; negative regulation of focal adhesion formation; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; post-translational protein modification; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; cellular protein metabolic process; negative regulation of dendritic cell antigen processing and presentation; response to hypoxia; immune response; sprouting angiogenesis; blood coagulation; positive regulation of phosphorylation; positive regulation of cell migration
Research Articles on TSP-1
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Product Notes
The Rat TSP-1 thbs1 (Catalog #AAA2501397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2501397 ELISA Kit recognizes Rat TSP-1. It is sometimes possible for the material contained within the vial of "TSP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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